Immunomodulatory Effects of Cinobufagin on Murine Lymphocytes and Macrophages
المؤلفون المشاركون
Yu, Yang
Wang, Hui
Meng, Xianhua
Hao, Lu
Fu, Yue
Fang, Linlin
Shen, Dan
Yu, Xiaomeng
Li, Jingshung
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Cinobufagin (CBG), a major bioactive component of the traditional Chinese medicine ChanSu, has been reported to have potent pharmacological activity.
In this study, we aimed to elucidate the effects of CBG on the activity of immune cells in mice.
Peritoneal macrophages and splenocytes from mice were prepared and cultured in RPMI1640 supplemented with 10% fetal bovine serum.
Concanavalin (ConA), lipopolysaccharide (LPS), and CBG (0.0125, 0.05, 0.15, or 0.25 μg/mL) were added to the culture medium, and the phagocytic activity of macrophages was detected by MTT assays.
Additionally, lymphocyte secretion of interleukin- (IL-)2 and IL-10 was detected by enzyme-linked immunosorbent assay, and the cell cycle distribution and cell surface markers were detected by flow cytometry.
Our results demonstrated that CBG promoted lymphocyte proliferation; this effect was suppressed by combined treatment with ConA or LPS.
Moreover, CBG also significantly improved the CD4+/CD8+ ratio in spleen lymphocytes and increased the percentage of spleen lymphocytes in the S phase.
Finally, we found that CBG enhanced the secretion of IL-2 and IL-10 and increased the phagocytosis ability of macrophages.
In summary, CBG could enhance activity of immune cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yu, Yang& Wang, Hui& Meng, Xianhua& Hao, Lu& Fu, Yue& Fang, Linlin…[et al.]. 2015. Immunomodulatory Effects of Cinobufagin on Murine Lymphocytes and Macrophages. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063666
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yu, Yang…[et al.]. Immunomodulatory Effects of Cinobufagin on Murine Lymphocytes and Macrophages. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1063666
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yu, Yang& Wang, Hui& Meng, Xianhua& Hao, Lu& Fu, Yue& Fang, Linlin…[et al.]. Immunomodulatory Effects of Cinobufagin on Murine Lymphocytes and Macrophages. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1063666
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063666
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر