Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species
المؤلفون المشاركون
Lin, Yajun
Zhen, Yong-Zhan
Li, Kai-Ji
Yang, Xiao-Shan
Zhao, Yu-Fang
Wei, Jie
Wei, Jing-Bo
Hu, Gang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-18
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Gambogic acid (GA) inhibits the proliferation of various human cancer cells.
However, because of its water insolubility, the antitumor efficacy of GA is limited.
Objectives.
To investigate the antitumor activity of gambogic acid lysinate (GAL) and its mechanism.
Methods.
Inhibition of cell proliferation was determined by MTT assay; intracellular ROS level was detected by staining cells with DCFH-DA; cell apoptosis was determined by flow cytometer and the mechanism of GAL was investigated by Western blot.
Results.
GAL inhibited the proliferation of MCF-7 cells with IC50 values 1.46 μmol/L comparable with GA (IC50, 1.16 μmol/L).
GAL promoted the production of ROS; however NAC could remove ROS and block the effect of GAL.
GAL inhibited the expression of SIRT1 but increased the phosphorylation of FOXO3a and the expression of p27Kip1.
At knockdown of FOXO3a, cell apoptosis induced by GAL can be partly blocked.
In addition it also enhanced the cleavage of caspase-3.
Conclusions.
GAL inhibited MCF-7 cell proliferation and induced MCF-7 cell apoptosis by increasing ROS level which could induce cell apoptosis by both SIRT1/FOXO3a/p27Kip1 and caspase-3 signal pathway.
These results suggested that GAL might be useful as a modulation agent in cancer chemotherapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhen, Yong-Zhan& Lin, Yajun& Li, Kai-Ji& Yang, Xiao-Shan& Zhao, Yu-Fang& Wei, Jie…[et al.]. 2015. Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063676
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhen, Yong-Zhan…[et al.]. Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063676
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhen, Yong-Zhan& Lin, Yajun& Li, Kai-Ji& Yang, Xiao-Shan& Zhao, Yu-Fang& Wei, Jie…[et al.]. Gambogic Acid Lysinate Induces Apoptosis in Breast Cancer MCF-7 Cells by Increasing Reactive Oxygen Species. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063676
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063676
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر