Xanthohumol Induces Growth Inhibition and Apoptosis in Ca Ski Human Cervical Cancer Cells
المؤلفون المشاركون
Abd Malek, Sri Nurestri
Yong, Wai Kuan
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-04-09
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
We investigate induction of apoptosis by xanthohumol on Ca Ski cervical cancer cell line.
Xanthohumol is a prenylated chalcone naturally found in hop plants, previously reported to be an effective anticancer agent in various cancer cell lines.
The present study showed that xanthohumol was effective to inhibit proliferation of Ca Ski cells based on IC50 values using sulforhodamine B (SRB) assay.
Furthermore, cellular and nuclear morphological changes were observed in the cells using phase contrast microscopy and Hoechst/PI fluorescent staining.
In addition, 48-hour long treatment with xanthohumol triggered externalization of phosphatidylserine, changes in mitochondrial membrane potential, and DNA fragmentation in the cells.
Additionally, xanthohumol mediated S phase arrest in cell cycle analysis and increased activities of caspase-3, caspase-8, and caspase-9.
On the other hand, Western blot analysis showed that the expression levels of cleaved PARP, p53, and AIF increased, while Bcl-2 and XIAP decreased in a dose-dependent manner.
Taken together, these findings indicate that xanthohumol-induced cell death might involve intrinsic and extrinsic apoptotic pathways, as well as downregulation of XIAP, upregulation of p53 proteins, and S phase cell cycle arrest in Ca Ski cervical cancer cells.
This work suggests that xanthohumol is a potent chemotherapeutic candidate for cervical cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yong, Wai Kuan& Abd Malek, Sri Nurestri. 2015. Xanthohumol Induces Growth Inhibition and Apoptosis in Ca Ski Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063804
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yong, Wai Kuan& Abd Malek, Sri Nurestri. Xanthohumol Induces Growth Inhibition and Apoptosis in Ca Ski Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1063804
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yong, Wai Kuan& Abd Malek, Sri Nurestri. Xanthohumol Induces Growth Inhibition and Apoptosis in Ca Ski Human Cervical Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063804
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063804
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر