Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study

المؤلفون المشاركون

Luo, Li-ke
Wei, Qing-jun
Liu, Lei
Zheng, Li
Zhao, Jin-min

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-02-23

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

As the main active constituent of Andrographis paniculata that was applied in treatment of many diseases including inflammation in ancient China, andrographolide (ANDRO) was found to facilitate reduction of edema and analgesia in arthritis.

This suggested that ANDRO may be promising anti-inflammatory agent to relieve destruction and degeneration of cartilage after inflammation.

In this study, the effect of ANDRO on rabbit articular chondrocytes in vitro was investigated.

Results showed that not more than 8 μM ANDRO did no harm to chondrocytes (P<0.05).

DNA content and glycosaminoglycan (GAG) /DNA were, respectively, improved in ANDRO groups comparing to the control (P<0.05).

ANDRO could promote expression of aggrecan, collagen II, and Sox9 genes while downregulating expression of collagen I gene (P<0.05).

Furthermore, hypertrophy that may result in chondrocyte ossification could not be detected in all groups (P>0.05).

The viability assay, hematoxylin-eosin, safranin O, and immunohistochemical staining also showed better performances in ANDRO groups.

As to the doses, 3 μM ANDRO showed the best performance.

The results indicate that ANDRO can accelerate proliferation of rabbit articular chondrocytes in vitro and meanwhile maintain the phenotype, which may provide valuable references for further exploration on arthritis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Luo, Li-ke& Wei, Qing-jun& Liu, Lei& Zheng, Li& Zhao, Jin-min. 2015. Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063868

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Luo, Li-ke…[et al.]. Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1063868

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Luo, Li-ke& Wei, Qing-jun& Liu, Lei& Zheng, Li& Zhao, Jin-min. Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1063868

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1063868