Quantification of Niacin and Its Metabolite Nicotinuric Acid in Human Plasma by LC-MSMS: Application to a Clinical Trial of a Fixed Dose Combination Tablet of Niacin Extended-ReleaseSimvastatin (500 mg10 mg) in Healthy Chinese Volunteers
المؤلفون المشاركون
Zhang, Pingping
Sun, Yantong
Shi, Guobing
Sui, Yin
Li, Qiuying
Tang, Yunbiao
Gu, Jingkai
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-08-05
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Our paper aimed to develop rapid, sensitive, and specific LC-MS/MS method for the quantification of niacin (NA) and its metabolite nicotinuric acid (NUA) in human plasma.
Following protein precipitation with acetonitrile, the NA, NUA, and internal standard (5-fluorouracil) were separated on a Zorbax 300SB-C8 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of methanol-2 mM ammonium acetate (3 : 97, v/v) at a flow rate of 1 mL/min (split 1 : 1).
A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated in negative ion mode.
The linear concentration ranges of the calibration curves were 5–800 ng/mL for NA and NUA.
The intra-assay RSD for quality control (QC) samples were from 5.0% to 8.7% for NA, and 5.5% to 7.6% for NUA.
The interassay RSD for QC samples were from 2.8% to 9.4% for NA, and 3.7% to 5.8% for NUA.
The relative errors for QC samples were from −2.2% to 2.3% for NA, and −0.6% to 3.2% for NUA.
The method was successfully applied to the investigation of the pharmacokinetic profiles of NA, NUA in human after single dose administration of Niacin extended-release/Simvastatin tablet (500 mg/10 mg).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Pingping& Sun, Yantong& Shi, Guobing& Sui, Yin& Li, Qiuying& Tang, Yunbiao…[et al.]. 2015. Quantification of Niacin and Its Metabolite Nicotinuric Acid in Human Plasma by LC-MSMS: Application to a Clinical Trial of a Fixed Dose Combination Tablet of Niacin Extended-ReleaseSimvastatin (500 mg10 mg) in Healthy Chinese Volunteers. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1064411
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Pingping…[et al.]. Quantification of Niacin and Its Metabolite Nicotinuric Acid in Human Plasma by LC-MSMS: Application to a Clinical Trial of a Fixed Dose Combination Tablet of Niacin Extended-ReleaseSimvastatin (500 mg10 mg) in Healthy Chinese Volunteers. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1064411
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Pingping& Sun, Yantong& Shi, Guobing& Sui, Yin& Li, Qiuying& Tang, Yunbiao…[et al.]. Quantification of Niacin and Its Metabolite Nicotinuric Acid in Human Plasma by LC-MSMS: Application to a Clinical Trial of a Fixed Dose Combination Tablet of Niacin Extended-ReleaseSimvastatin (500 mg10 mg) in Healthy Chinese Volunteers. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1064411
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1064411
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر