Stability-Indicating HPLC Determination of Gemcitabine in Pharmaceutical Formulations
المؤلفون المشاركون
Shakya, Ashok K.
Singh, Rahul
Naik, Rajashri
Shalan, Naeem
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-08
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
A simple, sensitive, inexpensive, and rapid stability indicating high performance liquid chromatographic method has been developed for determination of gemcitabine in injectable dosage forms using theophylline as internal standard.
Chromatographic separation was achieved on a Phenomenex Luna C-18 column (250 mm × 4.6 mm; 5μ) with a mobile phase consisting of 90% water and 10% acetonitrile (pH 7.00±0.05).
The signals of gemcitabine and theophylline were recorded at 275 nm.
Calibration curves were linear in the concentration range of 0.5–50 μg/mL.
The correlation coefficient was 0.999 or higher.
The limit of detection and limit of quantitation were 0.1498 and 0.4541 μg/mL, respectively.
The inter- and intraday precision were less than 2%.
Accuracy of the method ranged from 100.2% to 100.4%.
Stability studies indicate that the drug was stable to sunlight and UV light.
The drug gives 6 different hydrolytic products under alkaline stress and 3 in acidic condition.
Aqueous and oxidative stress conditions also degrade the drug.
Degradation was higher in the alkaline condition compared to other stress conditions.
The robustness of the methods was evaluated using design of experiments.
Validation reveals that the proposed method is specific, accurate, precise, reliable, robust, reproducible, and suitable for the quantitative analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Singh, Rahul& Shakya, Ashok K.& Naik, Rajashri& Shalan, Naeem. 2015. Stability-Indicating HPLC Determination of Gemcitabine in Pharmaceutical Formulations. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1064450
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Singh, Rahul…[et al.]. Stability-Indicating HPLC Determination of Gemcitabine in Pharmaceutical Formulations. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1064450
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Singh, Rahul& Shakya, Ashok K.& Naik, Rajashri& Shalan, Naeem. Stability-Indicating HPLC Determination of Gemcitabine in Pharmaceutical Formulations. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1064450
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1064450
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر