Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case
المؤلفون المشاركون
Mannocchi, Giulio
Pantano, Flaminia
Tittarelli, Roberta
Catanese, Miriam
Umani Ronchi, Federica
Busardò, Francesco Paolo
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-07
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
Introduction.
Clotiapine is an atypical antipsychotic of the dibenzothiazepine class introduced in a few European countries since 1970, efficient in treatment-resistant schizophrenic patients.
There is little published data on the therapeutic and toxic concentrations of this drug.
Aims.
The aim of the present study is the development and validation of a method that allows the detection and quantification of clotiapine in blood and urine specimens by gas chromatography-mass spectrometry (GC-MS).
Methods.
Validation was performed working on spiked postmortem blood and urine samples.
Samples were extracted with liquid-liquid extraction (LLE) technique at pH 8.5 with n-hexane/dichloromethane (85/15 v/v) and analysis was followed by GC-MS.
Methadone-d9 was used as internal standard.
Results.
The limit of detection (LOD) was 1.2 and 1.3 ng/mL for urine and blood, respectively, while the lower limit of quantification (LLOQ) was 3.9 and 4.3 ng/mL, respectively.
Linearity, precision, selectivity, accuracy, and recovery were also determined.
The method was applied to a postmortem case.
The blood and urine clotiapine concentrations were 1.32 and 0.49 μg/mL, respectively.
Conclusions.
A reliable GC-MS method for the detection and quantification of clotiapine in blood and urine samples has been developed and fully validated and then applied to a postmortem case.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mannocchi, Giulio& Pantano, Flaminia& Tittarelli, Roberta& Catanese, Miriam& Umani Ronchi, Federica& Busardò, Francesco Paolo. 2015. Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1064460
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mannocchi, Giulio…[et al.]. Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1064460
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mannocchi, Giulio& Pantano, Flaminia& Tittarelli, Roberta& Catanese, Miriam& Umani Ronchi, Federica& Busardò, Francesco Paolo. Development and Validation of a GC-MS Method for the Detection and Quantification of Clotiapine in Blood and Urine Specimens and Application to a Postmortem Case. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1064460
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1064460
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر