![](/images/graphics-bg.png)
Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay
المؤلفون المشاركون
Reischmann, Patricia
Fiebeck, Johanna
von der Weiden, Nadine
Müller, Oliver
المصدر
International Journal of Cell Biology
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-22
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The Wnt signaling pathway has been associated with many essential cell processes.
This study aims to examine the effects of Wnt signaling on proliferation of cultured HEK293T cells.
Cells were incubated with Wnt3a, and the activation of the Wnt pathway was followed by analysis of the level of the β-catenin protein and of the expression levels of the target genes MYC and CCND1.
The level of β-catenin protein increased up to fourfold.
While the mRNA levels of c-Myc and cyclin D1 increased slightly, the protein levels increased up to a factor of 1.5.
Remarkably, MTT and BrdU assays showed different results when measuring the proliferation rate of Wnt3a stimulated HEK293T cells.
In the BrdU assays an increase of the proliferation rate could be detected, which correlated to the applied Wnt3a concentration.
Oppositely, this correlation could not be shown in the MTT assays.
The MTT results, which are based on the mitochondrial activity, were confirmed by analysis of the succinate dehydrogenase complex by immunofluorescence and by western blotting.
Taken together, our study shows that Wnt3a activates proliferation of HEK293 cells.
These effects can be detected by measuring DNA synthesis rather than by measuring changes of mitochondrial activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Reischmann, Patricia& Fiebeck, Johanna& von der Weiden, Nadine& Müller, Oliver. 2015. Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay. International Journal of Cell Biology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1065361
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Reischmann, Patricia…[et al.]. Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay. International Journal of Cell Biology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1065361
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Reischmann, Patricia& Fiebeck, Johanna& von der Weiden, Nadine& Müller, Oliver. Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay. International Journal of Cell Biology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1065361
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1065361
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)