Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts
المؤلفون المشاركون
Zarattini, Guido
Mrak, Emanuela
Casati, Lavinia
Pagani, Francesca
Rubinacci, Alessandro
Sibilia, Valeria
المصدر
International Journal of Endocrinology
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Ghrelin, by binding growth hormone secretagogue receptor (GHS-R), promotes osteoblast proliferation but the signaling mechanism of GHS-R on these cells remains unclear.
Since canonical Wnt/β-catenin pathway is critically associated with bone homeostasis, we investigated its involvement in mediating ghrelin effects in osteoblasts and in osteoblast-osteoclast cross talk.
Ghrelin (10−10M) significantly increased β-catenin levels in rat osteoblasts (rOB).
This stimulatory action on β-catenin involves a specific interaction with GHS-R1a, as it is prevented by the selective GHS-R1a antagonist, D-Lys3-GHRP-6 (10−7M).
The effect of ghrelin on β-catenin involves the phosphorylation and inactivation of GSK-3β via protein kinase A (PKA).
Inhibition of PKA activity reduces the facilitatory action of ghrelin on β-catenin stabilization.
Ghrelin treatment of rOB significantly increases the expression of osteoprotegerin (OPG), which plays an important role in the regulation of osteoclastogenesis, and this effect is blocked by D-Lys3-GHRP-6.
Furthermore, ghrelin reduced RANKL/OPG ratio thus contrasting osteoclastogenesis.
Accordingly, conditioned media from rOB treated with ghrelin decreased the number of multinucleated TRAcP+ cells as compared with the conditioned media from untreated-control rOB.
Our data suggest new roles for ghrelin in modulating bone homeostasis via a specific interaction with GHSR-1a in osteoblasts with subsequent enhancement of both β-catenin levels and OPG expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mrak, Emanuela& Casati, Lavinia& Pagani, Francesca& Rubinacci, Alessandro& Zarattini, Guido& Sibilia, Valeria. 2015. Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts. International Journal of Endocrinology،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1065743
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mrak, Emanuela…[et al.]. Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts. International Journal of Endocrinology No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1065743
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mrak, Emanuela& Casati, Lavinia& Pagani, Francesca& Rubinacci, Alessandro& Zarattini, Guido& Sibilia, Valeria. Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts. International Journal of Endocrinology. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1065743
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1065743
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر