Challenges of the Unknown: Clinical Application of Microbial Metagenomics
المؤلفون المشاركون
Rose, Graham
Wooldridge, David J.
Anscombe, Catherine
Mee, Edward T.
Misra, Raju V.
Gharbia, Saheer
المصدر
International Journal of Genomics
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-09-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Availability of fast, high throughput and low cost whole genome sequencing holds great promise within public health microbiology, with applications ranging from outbreak detection and tracking transmission events to understanding the role played by microbial communities in health and disease.
Within clinical metagenomics, identifying microorganisms from a complex and host enriched background remains a central computational challenge.
As proof of principle, we sequenced two metagenomic samples, a known viral mixture of 25 human pathogens and an unknown complex biological model using benchtop technology.
The datasets were then analysed using a bioinformatic pipeline developed around recent fast classification methods.
A targeted approach was able to detect 20 of the viruses against a background of host contamination from multiple sources and bacterial contamination.
An alternative untargeted identification method was highly correlated with these classifications, and over 1,600 species were identified when applied to the complex biological model, including several species captured at over 50% genome coverage.
In summary, this study demonstrates the great potential of applying metagenomics within the clinical laboratory setting and that this can be achieved using infrastructure available to nondedicated sequencing centres.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rose, Graham& Wooldridge, David J.& Anscombe, Catherine& Mee, Edward T.& Misra, Raju V.& Gharbia, Saheer. 2015. Challenges of the Unknown: Clinical Application of Microbial Metagenomics. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1066008
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rose, Graham…[et al.]. Challenges of the Unknown: Clinical Application of Microbial Metagenomics. International Journal of Genomics No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1066008
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rose, Graham& Wooldridge, David J.& Anscombe, Catherine& Mee, Edward T.& Misra, Raju V.& Gharbia, Saheer. Challenges of the Unknown: Clinical Application of Microbial Metagenomics. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1066008
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1066008
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر