Development of Sensitive and Specific Analysis of Vildagliptin in Pharmaceutical Formulation by Gas Chromatography-Mass Spectrometry

المؤلف

Uçaktürk, Ebru

المصدر

Journal of Analytical Methods in Chemistry

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-11-22

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الكيمياء

الملخص EN

A sensitive and selective gas chromatography-mass spectrometry (GC-MS) method was developed and fully validated for the determination of vildagliptin (VIL) in pharmaceutical formulation.

Prior to GC-MS analysis, VIL was efficiently derivatized with MSTFA/NH4I/β-mercaptoethanol at 60°C for 30 min.

The obtained O-TMS derivative of VIL was detected by selected ion monitoring mode using the diagnostic ions m/z 223 and 252.

Nandrolone was chosen as internal standard.

The GC-MS method was fully validated by the following validation parameters: limit of detection (LOD) and quantitation (LOQ), linearity, precision, accuracy, specificity, stability, robustness, and ruggedness.

LOD and LOQ were found to be 1.5 and 3.5 ng mL−1, respectively.

The GC-MS method is linear in the range of 3.5–300 ng mL−1.

The intra- and interday precision values were less than ≤3.62%.

The intra- and interday accuracy values were found in the range of -0.26–2.06%.

Finally, the GC-MS method was successfully applied to determine VIL in pharmaceutical formulation.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Uçaktürk, Ebru. 2015. Development of Sensitive and Specific Analysis of Vildagliptin in Pharmaceutical Formulation by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067231

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Uçaktürk, Ebru. Development of Sensitive and Specific Analysis of Vildagliptin in Pharmaceutical Formulation by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1067231

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Uçaktürk, Ebru. Development of Sensitive and Specific Analysis of Vildagliptin in Pharmaceutical Formulation by Gas Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067231

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1067231