Development and Validation of an HPLC Method for Simultaneous Quantification of Clopidogrel Bisulfate, Its Carboxylic Acid Metabolite, and Atorvastatin in Human Plasma: Application to a Pharmacokinetic Study
المؤلفون المشاركون
Croitoru, Octavian
Spiridon, Adela-Maria
Belu, Ionela
Turcu-Ştiolică, Adina
Neamţu, Johny
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-29
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
A simple, sensitive, and specific reversed phase liquid chromatographic method was developed and validated for simultaneous quantification of clopidogrel, its carboxylic acid metabolite, and atorvastatin in human serum.
Plasma samples were deproteinized with acetonitrile and ibuprofen was chosen as internal standard.
Chromatographic separation was performed on an BDS Hypersil C18 column (250 × 4.6 mm; 5 μm) via gradient elution with mobile phase consisting of 10 mM phosphoric acid (sodium) buffer solution (pH = 2.6 adjusted with 85% orthophosphoric acid) : acetonitrile : methanol with flow rate of 1 mL·min−1.
Detection was achieved with PDA detector at 220 nm.
The method was validated in terms of linearity, sensitivity, precision, accuracy, limit of quantification, and stability tests.
Calibration curves of the analytes were found to be linear in the range of 0.008–2 μg·mL−1 for clopidogrel, 0.01–4 μg·mL−1 for its carboxylic acid metabolite, and 0.005–2.5 μg·mL−1 for atorvastatin.
The results of accuracy (as recovery) with ibuprofen as internal standard were in the range of 96–98% for clopidogrel, 94–98% for its carboxylic acid metabolite, and 90–99% for atorvastatin, respectively.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Croitoru, Octavian& Spiridon, Adela-Maria& Belu, Ionela& Turcu-Ştiolică, Adina& Neamţu, Johny. 2015. Development and Validation of an HPLC Method for Simultaneous Quantification of Clopidogrel Bisulfate, Its Carboxylic Acid Metabolite, and Atorvastatin in Human Plasma: Application to a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1067248
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Croitoru, Octavian…[et al.]. Development and Validation of an HPLC Method for Simultaneous Quantification of Clopidogrel Bisulfate, Its Carboxylic Acid Metabolite, and Atorvastatin in Human Plasma: Application to a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1067248
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Croitoru, Octavian& Spiridon, Adela-Maria& Belu, Ionela& Turcu-Ştiolică, Adina& Neamţu, Johny. Development and Validation of an HPLC Method for Simultaneous Quantification of Clopidogrel Bisulfate, Its Carboxylic Acid Metabolite, and Atorvastatin in Human Plasma: Application to a Pharmacokinetic Study. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1067248
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1067248
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر