Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation
المؤلفون المشاركون
Torbica, Aleksandra M.
Živančev, Dragan
Horvat, Daniela
Belović, Miona
Šimić, Gordana
Magdić, Damir
Đukić, Nevena
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-02-23
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types.
However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed.
On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins.
In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be improved in order to make its results more compliant with the results of RP-HPLC method.
Strong correlation (P≤0.001) was found between numbers of HMW glutenin peaks determined by LoaC and RP-HPLC methods.
Significant correlations (P≤0.05) were obtained between percentages of HMW and LMW glutenin subunits calculated with regard to total HMW + LMW area.
Even more significant correlation (P≤0.001) was found when percentages of individual HMW areas were calculated with regard to total HMW.
RP-HPLC method showed superiority in determination of gliadins since larger number and better resolution of gliadin peaks were obtained by this method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Živančev, Dragan& Horvat, Daniela& Torbica, Aleksandra M.& Belović, Miona& Šimić, Gordana& Magdić, Damir…[et al.]. 2015. Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. Journal of Chemistry،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1067459
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Živančev, Dragan…[et al.]. Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. Journal of Chemistry No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1067459
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Živančev, Dragan& Horvat, Daniela& Torbica, Aleksandra M.& Belović, Miona& Šimić, Gordana& Magdić, Damir…[et al.]. Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation. Journal of Chemistry. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1067459
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1067459
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر