Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells
المؤلفون المشاركون
Yamauchi, Akiyo
Itaya-Hironaka, Asako
Sakuramoto-Tsuchida, Sumiyo
Takeda, Maiko
Yoshimoto, Kiyomi
Miyaoka, Tomoko
Fujimura, Takanori
Tsujinaka, Hiroki
Tsuchida, Chikatsugu
Ota, Hiroyo
Takasawa, Shin
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-02-12
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Reg (Regenerating gene) gene was originally isolated from rat regenerating islets and its encoding protein was revealed as an autocrine/paracrine growth factor for β cells.
Rat Reg gene is activated in inflammatory conditions for β cell regeneration.
In human, although five functional REG family genes (REG Iα, REG Iβ, REG III, HIP/PAP, and REG IV) were isolated, their expressions in β cells under inflammatory conditions remained unclear.
In this study, we found that combined addition of IL-6 and dexamethasone (Dx) induced REG Iα and REG Iβ expression in human 1.1B4 β cells.
Promoter assay revealed that a signal transducer and activator of transcription- (STAT-) binding site in each promoter of REG Iα (TGCCGGGAA) and REG Iβ (TGCCAGGAA) was essential for the IL-6+Dx-induced promoter activation.
A Janus kinase 2 (JAK2) inhibitor significantly inhibited the IL-6+Dx-induced REG Iα and REG Iβ transcription.
Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that IL-6+Dx stimulation increased STAT3 binding to the REG Iα promoter.
Furthermore, small interfering RNA-mediated targeting of STAT3 blocked the IL-6+Dx-induced expression of REG Iα and REG Iβ.
These results indicate that the expression of REG Iα and REG Iβ should be upregulated in human β cells under inflammatory conditions through the JAK/STAT pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yamauchi, Akiyo& Itaya-Hironaka, Asako& Sakuramoto-Tsuchida, Sumiyo& Takeda, Maiko& Yoshimoto, Kiyomi& Miyaoka, Tomoko…[et al.]. 2015. Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1067842
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yamauchi, Akiyo…[et al.]. Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells. Journal of Diabetes Research No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1067842
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yamauchi, Akiyo& Itaya-Hironaka, Asako& Sakuramoto-Tsuchida, Sumiyo& Takeda, Maiko& Yoshimoto, Kiyomi& Miyaoka, Tomoko…[et al.]. Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1067842
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1067842
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر