Nuclear Factor of Activated T Cells Is Activated in the Endothelium of Retinal Microvessels in Diabetic Mice
المؤلفون المشاركون
Gomez, Maria F.
Zetterqvist, Anna V.
Blanco, Fabiana
Öhman, Jenny
Berglund, Lisa M.
de Frutos Garcia, Sergio
Al-Naemi, Raed
Wigren, Maria
McGuire, Paul G.
Gonzalez Bosc, Laura V.
Kotova, Olga
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-31
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The pathogenesis of diabetic retinopathy (DR) remains unclear but hyperglycemia is an established risk factor.
Endothelial dysfunction and changes in Ca2+ signaling have been shown to precede the onset of DR.
We recently demonstrated that high extracellular glucose activates the Ca2+/calcineurin-dependent transcription factor NFAT in cerebral arteries and aorta, promoting the expression of inflammatory markers.
Here we show, using confocal immunofluorescence, that NFAT is expressed in the endothelium of retinal microvessels and is readily activated by high glucose.
This was inhibited by the NFAT blocker A-285222 as well as by the ectonucleotidase apyrase, suggesting a mechanism involving the release of extracellular nucleotides.
Acute hyperglycemia induced by an IP-GTT (intraperitoneal glucose tolerance test) resulted in increased NFATc3 nuclear accumulation and NFAT-dependent transcriptional activity in retinal vessels of NFAT-luciferase reporter mice.
In both Akita (Ins2+/−) and streptozotocin- (STZ-) induced diabetic mice, NFAT transcriptional activity was elevated in retinal vessels.
In vivo inhibition of NFAT with A-285222 decreased the expression of OPN and ICAM-1 mRNA in retinal vessels, prevented a diabetes driven downregulation of anti-inflammatory IL-10 in retina, and abrogated the increased vascular permeability observed in diabetic mice.
Results identify NFAT signaling as a putative target for treatment of microvascular complications in diabetes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zetterqvist, Anna V.& Blanco, Fabiana& Öhman, Jenny& Kotova, Olga& Berglund, Lisa M.& de Frutos Garcia, Sergio…[et al.]. 2015. Nuclear Factor of Activated T Cells Is Activated in the Endothelium of Retinal Microvessels in Diabetic Mice. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1067907
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zetterqvist, Anna V.…[et al.]. Nuclear Factor of Activated T Cells Is Activated in the Endothelium of Retinal Microvessels in Diabetic Mice. Journal of Diabetes Research No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1067907
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zetterqvist, Anna V.& Blanco, Fabiana& Öhman, Jenny& Kotova, Olga& Berglund, Lisa M.& de Frutos Garcia, Sergio…[et al.]. Nuclear Factor of Activated T Cells Is Activated in the Endothelium of Retinal Microvessels in Diabetic Mice. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1067907
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1067907
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر