Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model
المؤلفون المشاركون
Soleimani, Masoud
Rahavi, Hossein
Hashemi, Seyed Mahmoud
Mohammadi, Jamal
Tajik, Nader
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Regenerative and immunomodulatory properties of mesenchymal stem cells (MSCs) might be applied for type 1 diabetes mellitus (T1DM) treatment.
Thus, we proposed in vitro assessment of adipose tissue-derived MSCs (AT-MSCs) immunomodulation on autoimmune response along with beta cell protection in streptozotocin- (STZ-) induced diabetic C57BL/6 mice model.
MSCs were extracted from abdominal adipose tissue of normal mice and cultured to proliferate.
Diabetic mice were prepared by administration of multiple low-doses of streptozotocin.
Pancreatic islets were isolated from normal mice and splenocytes prepared from normal and diabetic mice.
Proliferation, cytokine production, and insulin secretion assays were performed in coculture experiments.
AT-MSCs inhibited splenocytes proliferative response to specific (islet lysate) and nonspecific (PHA) triggers in a dose-dependent manner (P<0.05).
Decreased production of proinflammatory cytokines, such as IFN-γ, IL-2, and IL-17, and increased secretion of regulatory cytokines such as TGF-β, IL-4, IL-10, and IL-13 by stimulated splenocytes were also shown in response to islet lysate or PHA stimulants (P<0.05).
Finally, we demonstrated that AT-MSCs could effectively sustain viability as well as insulin secretion potential of pancreatic islets in the presence of reactive splenocytes (P<0.05).
In conclusion, it seems that MSCs may provide a new horizon for T1DM cell therapy and islet transplantation in the future.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rahavi, Hossein& Hashemi, Seyed Mahmoud& Soleimani, Masoud& Mohammadi, Jamal& Tajik, Nader. 2015. Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068019
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rahavi, Hossein…[et al.]. Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model. Journal of Diabetes Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068019
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rahavi, Hossein& Hashemi, Seyed Mahmoud& Soleimani, Masoud& Mohammadi, Jamal& Tajik, Nader. Adipose Tissue-Derived Mesenchymal Stem Cells Exert In Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068019
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068019
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر