fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

المؤلفون المشاركون

Hidalgo, María A.
Carretta, María D.
Teuber, Stefanie E.
Zárate, Cristian
Cárcamo, Leonardo
Concha, Ilona I.
Burgos, Rafael A.

المصدر

Journal of Immunology Research

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-11-08

دولة النشر

مصر

عدد الصفحات

14

التخصصات الرئيسية

الأحياء

الملخص EN

N-Formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF) induce similar intracellular signalling profiles; but only fMLP induces interleukin-8 (IL-8) release and nicotinamide adenine dinucleotide phosphate reduced (NADPH) oxidase activity in neutrophils.

Because the role of ROS on IL-8 release in neutrophils is until now controversial, we assessed if NADPH oxidase is involved in the IL-8 secretions and PI3K/Akt, MAPK, and NF-κB pathways activity induced by fMLP.

Neutrophils were obtained from healthy volunteers.

IL-8 was measured by ELISA, IL-8 mRNA by qPCR, and ROS production by luminol-amplified chemiluminescence, reduction of ferricytochrome c, and FACS.

Intracellular pH changes were detected by spectrofluorescence.

ERK1/2, p38 MAPK, and Akt phosphorylation were analysed by immunoblotting and NF-κB was analysed by immunocytochemistry.

Hydroxy-3-methoxyaceto-phenone (HMAP), diphenyleneiodonium (DPI), and siRNA Nox2 reduced the ROS and IL-8 release in neutrophils treated with fMLP.

HMAP, DPI, and amiloride (a Na+/H+ exchanger inhibitor) inhibited the Akt phosphorylation and did not affect the p38 MAPK and ERK1/2 activity.

DPI and HMAP reduced NF-κB translocation induced by fMLP.

We showed that IL-8 release induced by fMLP is dependent on NADPH oxidase, and ROS could play a redundant role in cell signalling, ultimately activating the PI3K/Akt and NF-κB pathways in neutrophils.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Hidalgo, María A.& Carretta, María D.& Teuber, Stefanie E.& Zárate, Cristian& Cárcamo, Leonardo& Concha, Ilona I.…[et al.]. 2015. fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1068359

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Hidalgo, María A.…[et al.]. fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils. Journal of Immunology Research No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1068359

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Hidalgo, María A.& Carretta, María D.& Teuber, Stefanie E.& Zárate, Cristian& Cárcamo, Leonardo& Concha, Ilona I.…[et al.]. fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1068359

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1068359