A Novel PET Imaging Using 64Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells
المؤلفون المشاركون
Kobayashi, Kazuko
Sasaki, Takanori
Takenaka, Fumiaki
Yakushiji, Hiromasa
Fujii, Yoshihiro
Kishi, Yoshiro
Kita, Shoichi
Shen, Lianhua
Kumon, Hiromi
Matsuura, Eiji
المصدر
Journal of Immunology Research
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-25
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Mesothelin (MSLN) is a 40-kDa cell differentiation-associated glycoprotein appearing with carcinogenesis and is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas, while its expression in normal tissue is limited to mesothelial cells lining the pleura, pericardium, and peritoneum.
Clone 11-25 is a murine hybridoma secreting monoclonal antibody (mAb) against human MSLN.
In this study, we applied the 11-25 mAb to in vivo imaging to detect MSLN-expressing tumors.
In in vitro and ex vivo immunochemical studies, we demonstrated specificity of 11-25 mAb to membranous MSLN expressed on several pancreatic cancer cells.
We showed the accumulation of Alexa Fluor 750-labeled 11-25 mAb in MSLN-expressing tumor xenografts in athymic nude mice.
Then, 11-25 mAb was labeled with 64Cu via a chelating agent DOTA and was used in both in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice.
We confirmed that 64Cu-labeled 11-25 mAb highly accumulated in MSLN-expressing tumors as compared to MSLN-negative ones.
The 64Cu-labeled 11-25 mAb is potentially useful as a PET probe capable of being used for wide range of tumors, rather than 18F-FDG that occasionally provides nonspecific accumulation into the inflammatory lesions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kobayashi, Kazuko& Sasaki, Takanori& Takenaka, Fumiaki& Yakushiji, Hiromasa& Fujii, Yoshihiro& Kishi, Yoshiro…[et al.]. 2015. A Novel PET Imaging Using 64Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1068425
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kobayashi, Kazuko…[et al.]. A Novel PET Imaging Using 64Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells. Journal of Immunology Research No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1068425
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kobayashi, Kazuko& Sasaki, Takanori& Takenaka, Fumiaki& Yakushiji, Hiromasa& Fujii, Yoshihiro& Kishi, Yoshiro…[et al.]. A Novel PET Imaging Using 64Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1068425
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068425
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر