NK and NKT Cell Depletion Alters the Outcome of Experimental Pneumococcal Pneumonia: Relationship with Regulation of Interferon-γ Production
المؤلفون المشاركون
Giamarellos-Bourboulis, Evangelos J.
Christaki, Eirini
Papadopoulou, Nikoletta
Pistiki, Aikaterini
Droggiti, Dionysia-Irini
Georgitsi, Marianna
Machova, Alzbeta
Lambrelli, Dimitra
Malisiovas, Nicolaos
Opal, Steven M.
Diza, Evdoxia
Nikolaidis, Pavlos
المصدر
Journal of Immunology Research
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Natural killer (NK) and natural killer T (NKT) cells contribute to the innate host defense but their role in bacterial sepsis remains controversial.
Methods.
C57BL/6 mice were infected intratracheally with 5 × 105 cfu of Streptococcus pneumoniae.
Animals were divided into sham group (Sham); pretreated with isotype control antibody (CON) group; pretreated with anti-asialo GM1 antibody (NKd) group; and pretreated with anti-CD1d monoclonal antibody (NKTd) group before bacterial challenge.
Serum and tissue samples were analyzed for bacterial load, cytokine levels, splenocyte apoptosis rates, and cell characteristics by flow cytometry.
Splenocyte miRNA expression was also analyzed and survival was assessed.
Results.
NK cell depletion prolonged survival.
Upon inhibition of NKT cell activation, spleen NK (CD3−/NK1.1+) cells increased compared to all other groups.
Inhibition of NKT cell activation led to higher bacterial loads and increased levels of serum and splenocyte IFN-γ.
Splenocyte miRNA analysis showed that miR-200c and miR-29a were downregulated, while miR-125a-5p was upregulated, in anti-CD1d treated animals.
These changes were moderate after NK cell depletion.
Conclusions.
NK cells appear to contribute to mortality in pneumococcal pneumonia.
Inhibition of NKT cell activation resulted in an increase in spleen NK (CD3−/NK1.1+) cells and a higher IFN-γ production, while altering splenocyte miRNA expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Christaki, Eirini& Diza, Evdoxia& Giamarellos-Bourboulis, Evangelos J.& Papadopoulou, Nikoletta& Pistiki, Aikaterini& Droggiti, Dionysia-Irini…[et al.]. 2015. NK and NKT Cell Depletion Alters the Outcome of Experimental Pneumococcal Pneumonia: Relationship with Regulation of Interferon-γ Production. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068511
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Christaki, Eirini…[et al.]. NK and NKT Cell Depletion Alters the Outcome of Experimental Pneumococcal Pneumonia: Relationship with Regulation of Interferon-γ Production. Journal of Immunology Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068511
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Christaki, Eirini& Diza, Evdoxia& Giamarellos-Bourboulis, Evangelos J.& Papadopoulou, Nikoletta& Pistiki, Aikaterini& Droggiti, Dionysia-Irini…[et al.]. NK and NKT Cell Depletion Alters the Outcome of Experimental Pneumococcal Pneumonia: Relationship with Regulation of Interferon-γ Production. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068511
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068511
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر