Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy
المؤلفون المشاركون
Dannull, Jens
Tan, Chunrui
Farrell, Christine
Wang, Cynthia
Pruitt, Scott
Nair, Smita K.
Lee, Walter T.
المصدر
Journal of Immunology Research
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-28
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Dendritic cell- (DC-) tumor fusion cells stimulate effective in vivo antitumor responses.
However, therapeutic approaches are dependent upon the coadministration of exogenous 3rd signals.
The purpose of this study was to determine the mechanisms for inadequate 3rd signaling by electrofused DC-tumor cell hybrids.
Methods.
Murine melanoma cells were fused with DCs derived from C57BL/6 mice.
Quantitative real-time PCR (qPCR) was used to determine relative changes in Th (T helper) 1 and Th2 cytokine gene expression.
In addition, changes in gene expression of fusion cells were determined by microarray.
Last, cytokine secretion by fusion cells upon inhibition of signaling pathways was analyzed by ELISA.
Results.
qPCR analyses revealed that fusion cells exhibited a downregulation of Th1 associated cytokines IL-12 and IL-15 and an upregulation of the Th2 cytokine IL-4.
Microarray studies further showed that the expression of chemokines, costimulatory molecules, and matrix-metalloproteinases was deregulated in fusion cells.
Lastly, inhibitor studies demonstrate that inhibition of the PI3K/Akt/mTOR signaling pathway could restore the secretion of bioactive IL-12p70 by fusion cells.
Conclusion.
Our results suggest that combining fusion cell-based vaccination with administration of inhibitors of the PI3K/Akt/mTOR signaling pathway may enhance antitumor responses in patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dannull, Jens& Tan, Chunrui& Farrell, Christine& Wang, Cynthia& Pruitt, Scott& Nair, Smita K.…[et al.]. 2015. Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068596
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dannull, Jens…[et al.]. Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy. Journal of Immunology Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068596
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dannull, Jens& Tan, Chunrui& Farrell, Christine& Wang, Cynthia& Pruitt, Scott& Nair, Smita K.…[et al.]. Gene Expression Profile of Dendritic Cell-Tumor Cell Hybrids Determined by Microarrays and Its Implications for Cancer Immunotherapy. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068596
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068596
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر