Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA1 Mice
المؤلفون المشاركون
Collins, Fraser L.
Williams, Jessica O.
Bloom, Anja C.
Stone, Michael D.
Choy, Ernest
Wang, Eddie C. Y.
Williams, Anwen S.
المصدر
Journal of Immunology Research
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-03
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objectives.
Genome wide association studies identified TNFSF member TNF-like protein 1A (TL1A, TNFSF15) as a potential modulator of ankylosing spondylitis (AS).
TL1A is the only confirmed TNFSF ligand of death receptor 3 (DR3, TNFRSF25); however, its role in disease pathology is not characterised.
We evaluated DR3’s role in controlling osteoblast- (OB-) dependent bone formation in vitro and in vivo.
Methods.
Osteoprogenitor cells and OB were cultured from male DR3-deficient (DR3ko) and wild-type (DR3wt) DBA/1 mice.
DR3 and RANKL expression were tested by flow cytometry.
Alkaline phosphatase and mineralization were quantified.
Osteopontin, osteoprotegerin, and pro MMP-9 were measured by ELISA.
A fluorescent probe (BoneTag) was used to measure in vivo mineralization in 10-month-old mice.
Results.
DR3 was expressed on osteoprogenitors and OB from DR3wt mice.
Alkaline phosphatase, osteopontin, and mineral apposition were significantly elevated in DR3wt cultures.
Levels of RANKL were comparable whilst osteoprotegerin was significantly increased in DR3wt cultures.
In vivo incorporation of BoneTag was significantly lower in the thoracic vertebrae of 10-month-old DR3ko mice.
Conclusions.
These data identify new roles for DR3 in regulating OB-dependent bone mineral apposition.
They potentially begin to explain the atypical pattern of new bone formation observed in the axial skeleton of grouped, aging DBA/1 mice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Collins, Fraser L.& Williams, Jessica O.& Bloom, Anja C.& Stone, Michael D.& Choy, Ernest& Wang, Eddie C. Y.…[et al.]. 2015. Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA1 Mice. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068631
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Collins, Fraser L.…[et al.]. Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA1 Mice. Journal of Immunology Research No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1068631
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Collins, Fraser L.& Williams, Jessica O.& Bloom, Anja C.& Stone, Michael D.& Choy, Ernest& Wang, Eddie C. Y.…[et al.]. Death Receptor 3 (TNFRSF25) Increases Mineral Apposition by Osteoblasts and Region Specific New Bone Formation in the Axial Skeleton of Male DBA1 Mice. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068631
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068631
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر