Synthesis and Characterization of an Amphiphilic Linoleic Acid-g-Quaternary Chitosan with Low Toxicity

الملخص EN

A novel amphiphilic derivative of chitosan, namely, a linoleic acid-g-quaternary chitosan (LA-g-QC), was designed and synthesized as low toxic material for biomedical applications in this study.

The chemical structure of LA-g-QC was characterized by Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H-NMR), and elemental analysis.

LA-g-QC could form nanosized micelles with self-assembly, which was confirmed by the results of critical micelle concentration (CMC) via fluorescence spectroscopy.

The average size of LA-g-QC was 140 nm and its zeta potential was approximately +35.50 mV.

CMC value was 31.00 mg/mL.

Furthermore, LA-g-QC micelles, at final concentrations between 0.94 μg/mL and 30 μg/mL, did not inhibit the proliferation of HepG2 or SMMC 7721 cell lines.

Taken together, LA-g-QC has low cytotoxicity and high potential for the preparation of novel drug-delivery micelles.