Cytotoxicity and Biological Efficacy of Exendin-4-Encapsulated Solid Lipid Nanoparticles in INS-1 Cells

الملخص EN

Exendin-4 (Ex-4), a peptide of glucagon-like peptide-1 receptor agonist, is a potent insulinotropic agent and alternative drug delivery systems to increase therapeutic utility have been explored.

We developed exendin-4-encapsulated solid lipid nanoparticles (Eudragit Ex-4 SLNs) and compared the effects of Eudragit Ex-4 SLNs with those of native Ex-4 on INS-1 cells.

We observed no significant toxic effects of nanoparticles at concentrations from 1 nM to 100 nM.

Similar to Ex-4, Eudragit Ex-4 SLNs stimulated the production of cyclic AMP at 10 nM.

Moreover, unlike treatment with the vehicle, treatment with 10 nM Eudragit Ex-4 SLNs increased insulin mRNA levels and insulin secretion.

These insulinotropic effects of Eudragit Ex-4 SLNs were comparable to those of Ex-4.

Thus, our in vitro results suggest that the biological effects of Eudragit Ex-4 SLNs are similar to those of Ex-4, and further in vivo pharmacokinetic studies are required to propose an alternative sustained release drug system.