Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17

الملخص EN

Purpose.

To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1β, IL-18, and IL-17A.

Materials and Methods.

RSCs were cultured in a specific culture medium and were treated with these cytokines.

Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL-17rc and proapoptotic proteins was detected by immunocytochemistry and expression of Il-6 and Il-17a was detected by quantitative RT-PCR.

As a comparison, primary mouse retinal pigment epithelium (RPE) cells were also treated with IL-1β, IL-18, or IL-17A and analyzed for the expression of Il-6 and Il-17rc.

Results.

Treatment with IL-1β, IL-18, or IL-17A decreased RSC viability in a dose-dependent fashion and led to damage in cellular ultrastructure including pyroptotic and/or necroptotic cells.

IL-1β and IL-18 could induce proapoptotic protein expression.

All treatments induced significantly higher expression of Il-6 and Il-17rc in both cells.

However, neither IL-1β nor IL-18 could induce Il-17a expression in RSCs.

Conclusions.

IL-1β, IL-18, and IL-17A induce retinal cell death via pyroptosis/necroptosis and apoptosis.

They also provoke proinflammatory responses in RSCs.

Though IL-1β and IL-18 could not induce Il-17a expression in RSCs, they both increase Il-17rc expression, which may mediate the effect of Il-17a.