Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17
Joint Authors
Chen, Shida
Shen, Defen
Popp, Nicholas A.
Ogilvy, Alexander J.
Tuo, Jingsheng
Abu-Asab, Mones
Xie, Ting
Chan, Chi-Chao
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1β, IL-18, and IL-17A.
Materials and Methods.
RSCs were cultured in a specific culture medium and were treated with these cytokines.
Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL-17rc and proapoptotic proteins was detected by immunocytochemistry and expression of Il-6 and Il-17a was detected by quantitative RT-PCR.
As a comparison, primary mouse retinal pigment epithelium (RPE) cells were also treated with IL-1β, IL-18, or IL-17A and analyzed for the expression of Il-6 and Il-17rc.
Results.
Treatment with IL-1β, IL-18, or IL-17A decreased RSC viability in a dose-dependent fashion and led to damage in cellular ultrastructure including pyroptotic and/or necroptotic cells.
IL-1β and IL-18 could induce proapoptotic protein expression.
All treatments induced significantly higher expression of Il-6 and Il-17rc in both cells.
However, neither IL-1β nor IL-18 could induce Il-17a expression in RSCs.
Conclusions.
IL-1β, IL-18, and IL-17A induce retinal cell death via pyroptosis/necroptosis and apoptosis.
They also provoke proinflammatory responses in RSCs.
Though IL-1β and IL-18 could not induce Il-17a expression in RSCs, they both increase Il-17rc expression, which may mediate the effect of Il-17a.
American Psychological Association (APA)
Chen, Shida& Shen, Defen& Popp, Nicholas A.& Ogilvy, Alexander J.& Tuo, Jingsheng& Abu-Asab, Mones…[et al.]. 2015. Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1069710
Modern Language Association (MLA)
Chen, Shida…[et al.]. Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17. Journal of Ophthalmology No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1069710
American Medical Association (AMA)
Chen, Shida& Shen, Defen& Popp, Nicholas A.& Ogilvy, Alexander J.& Tuo, Jingsheng& Abu-Asab, Mones…[et al.]. Responses of Multipotent Retinal Stem Cells to IL-1β, IL-18, or IL-17. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1069710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069710