Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract

المؤلفون المشاركون

Zhu, Xi
Zhang, Guowei
Kang, Lihua
Guan, Huaijin

المصدر

Journal of Ophthalmology

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-10-05

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Purpose.

To examine the promoter methylation and histone modification of WRN (Werner syndrome gene), a DNA repair gene, and their relationship with the gene expression in age-related cataract (ARC) lens.

Methods.

We collected the lenses after cataract surgery from 117ARC patients and 39 age-matched non-ARC.

WRN expression, DNA methylation and histone modification around the CpG island were assessed.

The methylation status of Human-lens-epithelium cell (HLEB-3) was chemically altered to observe the relationship between methylation and expression of WRN.

Results.

The WRN expression was significantly decreased in the ARC anterior lens capsules comparing with the control.

The CpG island of WRN promoter in the ARC anterior lens capsules displayed hypermethylation comparing with the controls.

The WRN promoter was almost fully methylated in the cortex of ARC and control lens.

Acetylated H3 was lower while methylated H3-K9 was higher in ARC anterior lens capsules than that of the controls.

The expression of WRN in HLEB-3 increased after demethylation of the cells.

Conclusions.

A hypermethylation in WRN promoter and altered histone modification in anterior lens capsules might contribute to the ARC mechanism.

The data suggest an association of altered DNA repair capability in lens with ARC pathogenesis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhu, Xi& Zhang, Guowei& Kang, Lihua& Guan, Huaijin. 2015. Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract. Journal of Ophthalmology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069777

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhu, Xi…[et al.]. Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract. Journal of Ophthalmology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069777

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhu, Xi& Zhang, Guowei& Kang, Lihua& Guan, Huaijin. Epigenetic Regulation of Werner Syndrome Gene in Age-Related Cataract. Journal of Ophthalmology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069777

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1069777