Identification of Anti-Long Chain Saturated Fatty Acid IgG Antibodies in Serum of Patients with Type 2 Diabetes

الملخص EN

High levels of serum long chain saturated fatty acids (LCSFAs) have been associated with inflammation in type 2 diabetes.

Dietary SFAs can promote inflammation, the secretion of IgG antibodies, and secretion of the proinflammatory cytokine IL-1β.

This study characterizes anti-LCSFA IgG antibodies from patients with type 2 diabetes.

Serum samples from several cohorts with type 2 diabetes were analyzed for the presence of anti-LCSFA IgG, the cytokine IL-1β, and nonesterified fatty acids.

Anti-LCSFA IgG was isolated from patient samples and used for in vitro characterization of avidity and specificity.

A cohort participating in En Balance, a diabetes health education program that improved diabetes management, tested positive for anti-LCSFA IgG.

Following the 3-month program, the cohort showed a significant reduction in anti-LCSFA IgG levels.

Anti-LCSFA antibodies isolated from these patients demonstrated high avidity, were specific for long chain SFAs, and correlated with serum fatty acids in patients with managed type 2 diabetes.

Interestingly, anti-LCSFA IgG neutralized PA-induced IL-1β secretion by dendritic cells.

Our data shows that nonesterified SFAs are recognized by IgG antibodies present in human blood.

The identification of anti-LCSFA IgG antibodies in human sera establishes a basis for further exploration of lipid induced immune responses in diabetic patients.