Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging
المؤلفون المشاركون
Boddicker, Melissa A.
DeMaula, Christopher
Bednar, Bohumil
Heinrichs, Jon H.
Smith, Jeffrey G.
Connolly, Brett
Patel, Manishkumar
Lin, Shu-An
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Chlamydia trachomatis is a bacterial sexually transmitted disease with over 1.3 million cases reported to the CDC in 2010.
While Chlamydia infection is easily treated with antibiotics, up to 70% of infections are asymptomatic and go untreated.
The current mouse model relies on invasive upper genital tract gross pathology readouts at ~60–80 days postinfection.
High throughput optical imaging through the use of biomarkers has been successfully used to quickly evaluate several disease processes.
Here we evaluate Neutrophil Elastase 680 (Elastase680) for its ability to measure Chlamydia muridarum associated inflammation in live mice using fluorescence molecular tomography (FMT) and In Vivo Imaging System (IVIS).
Optical imaging was able to distinguish with statistical significance between vaccinated and nonvaccinated mice as well as mock-challenged and challenged mice 2 weeks after challenge which was 9 weeks sooner than typical gross pathological assessment.
Immunohistochemistry confirmed the presence of neutrophils and correlated well with both in vivo and ex vivo imaging.
In this report we demonstrate that Elastase680 can be used as a molecular imaging biomarker for inflammation associated with chlamydial infection in a mouse model and that these biomarkers can significantly decrease the time for pathology evaluation and thus increase the rate of therapeutics discovery.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Patel, Manishkumar& Lin, Shu-An& Boddicker, Melissa A.& DeMaula, Christopher& Connolly, Brett& Bednar, Bohumil…[et al.]. 2015. Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072216
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Patel, Manishkumar…[et al.]. Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging. Mediators of Inflammation No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1072216
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Patel, Manishkumar& Lin, Shu-An& Boddicker, Melissa A.& DeMaula, Christopher& Connolly, Brett& Bednar, Bohumil…[et al.]. Quantitative In Vivo Detection of Chlamydia muridarum Associated Inflammation in a Mouse Model Using Optical Imaging. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1072216
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072216
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر