Punicalagin Induces Nrf2HO-1 Expression via Upregulation of PI3KAKT Pathway and Inhibits LPS-Induced Oxidative Stress in RAW264.7 Macrophages
المؤلفون المشاركون
Xu, Xiaolong
Li, Hongquan
Hou, Xiaolin
Li, Deyin
He, Shasha
Wan, Changrong
Yin, Peng
Liu, Mingjiang
Liu, Fenghua
Xu, Jianqin
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-04-19
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Reactive oxygen species (ROS) and oxidative stress are thought to play a central role in potentiating macrophage activation, causing excessive inflammation, tissue damage, and sepsis.
Recently, we have shown that punicalagin (PUN) exhibits anti-inflammatory activity in LPS-stimulated macrophages.
However, the potential antioxidant effects of PUN in macrophages remain unclear.
Revealing these effects will help understand the mechanism underlying its ability to inhibit excessive macrophage activation.
Hemeoxygenase-1 (HO-1) exhibits antioxidant activity in macrophages.
Therefore, we hypothesized that HO-1 is a potential target of PUN and tried to reveal its antioxidant mechanism.
Here, PUN treatment increased HO-1 expression together with its upstream mediator nuclear factor-erythroid 2 p45-related factor 2 (Nrf2).
However, specific inhibition of Nrf2 by brusatol (a specific Nrf2 inhibitor) dramatically blocked PUN-induced HO-1 expression.
Previous research has demonstrated that the PI3K/Akt pathway plays a critical role in modulating Nrf2/HO-1 protein expression as an upstream signaling molecule.
Here, LY294002, a specific PI3K/Akt inhibitor, suppressed PUN-induced HO-1 expression and led to ROS accumulation in macrophages.
Furthermore, PUN inhibited LPS-induced oxidative stress in macrophages by reducing ROS and NO generation and increasing superoxide dismutase (SOD) 1 mRNA expression.
These findings provide new perspectives for novel therapeutic approaches using antioxidant medicines and compounds against oxidative stress and excessive inflammatory diseases including tissue damage, sepsis, and endotoxemic shock.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Xiaolong& Li, Hongquan& Hou, Xiaolin& Li, Deyin& He, Shasha& Wan, Changrong…[et al.]. 2015. Punicalagin Induces Nrf2HO-1 Expression via Upregulation of PI3KAKT Pathway and Inhibits LPS-Induced Oxidative Stress in RAW264.7 Macrophages. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072296
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Xiaolong…[et al.]. Punicalagin Induces Nrf2HO-1 Expression via Upregulation of PI3KAKT Pathway and Inhibits LPS-Induced Oxidative Stress in RAW264.7 Macrophages. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072296
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Xiaolong& Li, Hongquan& Hou, Xiaolin& Li, Deyin& He, Shasha& Wan, Changrong…[et al.]. Punicalagin Induces Nrf2HO-1 Expression via Upregulation of PI3KAKT Pathway and Inhibits LPS-Induced Oxidative Stress in RAW264.7 Macrophages. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072296
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072296
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر