Construction, Expression, and Characterization of a Recombinant Immunotoxin Targeting EpCAM
المؤلفون المشاركون
Lv, Minghua
Qiu, Feng
Li, Tingting
Sun, Yuanjie
Zhang, Chunmei
Zhu, Ping
Qi, Xiaokun
Wan, Jun
Yang, Kun
Zhang, Kui
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-04-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Epithelial cell adhesion molecule (EpCAM) is a type I transmembrane glycoprotein overexpressed in human epithelioma but with relatively low expression in normal epithelial tissues.
To exploit this differential expression pattern for targeted cancer therapy, an EpCAM-targeted immunotoxin was developed and its antitumor activity was investigated in vitro.
An immunotoxin (scFv2A9-PE or APE) was constructed by genetically fusing a truncated form (PE38KDEL) of Pseudomonas aeruginosa exotoxin with an anti-EpCAM single-chain variable fragment (scFv).
ELISA and flow cytometry were performed to verify immunotoxin (scFv2A9-PE or APE) antigen-binding activity with EpCAM.
Cytotoxicity was measured by MTT assay.
Confocal microscopy was used to observe its cellular localization.
The results of ELISA and flow cytometry revealed that the immunotoxin efficiently recognized recombinant and natural EpCAM.
Its antigen-binding activity was relatively lower than 2A9.
MTT assay confirmed potent reduction in EpCAM-positive HHCC (human hepatocellular carcinoma) cell viability (IC50 50 pM).
Immunofluorescence revealed that the immunotoxin localized to endoplasmic reticulum 24 h later.
In conclusion, we described the development of an EpCAM-targeted immunotoxin with potent activity against tumor cells, which may lay the foundation for future development of therapeutic antibody for the treatment of EpCAM-positive tumors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lv, Minghua& Qiu, Feng& Li, Tingting& Sun, Yuanjie& Zhang, Chunmei& Zhu, Ping…[et al.]. 2015. Construction, Expression, and Characterization of a Recombinant Immunotoxin Targeting EpCAM. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072365
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lv, Minghua…[et al.]. Construction, Expression, and Characterization of a Recombinant Immunotoxin Targeting EpCAM. Mediators of Inflammation No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1072365
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lv, Minghua& Qiu, Feng& Li, Tingting& Sun, Yuanjie& Zhang, Chunmei& Zhu, Ping…[et al.]. Construction, Expression, and Characterization of a Recombinant Immunotoxin Targeting EpCAM. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1072365
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072365
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر