The In Vivo Granulopoietic Response to Dexamethasone Injection Is Abolished in Perforin-Deficient Mutant Mice and Corrected by Lymphocyte Transfer from Nonsensitized Wild-Type Donors
المؤلفون المشاركون
Xavier-Elsas, P.
Silva, C. L. C. A.
Vieira, B. M.
De Luca, B.
Masid-de-Brito, D.
Gaspar-Elsas, M. I.
Queto, Túlio
Vieira, Thiago Soares de Souza
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-04
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Exogenously administered glucocorticoids enhance eosinophil and neutrophil granulocyte production from murine bone-marrow.
A hematological response dependent on endogenous glucocorticoids underlies bone-marrow eosinophilia induced by trauma or allergic sensitization/challenge.
We detected a defect in granulopoiesis in nonsensitized, perforin-deficient mice.
In steady-state conditions, perforin- (Pfp-) deficient mice showed significantly decreased bone-marrow and blood eosinophil and neutrophil counts, and colony formation in response to GM-CSF, relative to wild-type controls of comparable age and/or weight.
By contrast, peripheral blood or spleen total cell and lymphocyte numbers were not affected by perforin deficiency.
Dexamethasone enhanced colony formation by GM-CSF-stimulated progenitors from wild-type controls, but not Pfp mice.
Dexamethasone injection increased bone-marrow eosinophil and neutrophil counts in wild-type controls, but not Pfp mice.
Because perforin is expressed in effector lymphocytes, we examined whether this defect would be corrected by transferring wild-type lymphocytes into perforin-deficient recipients.
Short-term reconstitution of the response to dexamethasone was separately achieved for eosinophils and neutrophils by transfer of distinct populations of splenic lymphocytes from nonsensitized wild-type donors.
Transfer of the same amount of splenic lymphocytes from perforin-deficient donors was ineffective.
This demonstrates that the perforin-dependent, granulopoietic response to dexamethasone can be restored by transfer of innate lymphocyte subpopulations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xavier-Elsas, P.& Silva, C. L. C. A.& Vieira, B. M.& Masid-de-Brito, D.& Queto, Túlio& De Luca, B.…[et al.]. 2015. The In Vivo Granulopoietic Response to Dexamethasone Injection Is Abolished in Perforin-Deficient Mutant Mice and Corrected by Lymphocyte Transfer from Nonsensitized Wild-Type Donors. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1072387
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xavier-Elsas, P.…[et al.]. The In Vivo Granulopoietic Response to Dexamethasone Injection Is Abolished in Perforin-Deficient Mutant Mice and Corrected by Lymphocyte Transfer from Nonsensitized Wild-Type Donors. Mediators of Inflammation No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1072387
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xavier-Elsas, P.& Silva, C. L. C. A.& Vieira, B. M.& Masid-de-Brito, D.& Queto, Túlio& De Luca, B.…[et al.]. The In Vivo Granulopoietic Response to Dexamethasone Injection Is Abolished in Perforin-Deficient Mutant Mice and Corrected by Lymphocyte Transfer from Nonsensitized Wild-Type Donors. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1072387
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072387
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر