Regulation of System xc- by Pharmacological Manipulation of Cellular Thiols
المؤلفون المشاركون
Albano, Rebecca
Raddatz, Nicholas J.
Hjelmhaug, Julie
Baker, David A.
Lobner, Doug
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-04-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The cystine/glutamate exchanger (system xc-) mediates the transport of cystine into the cell in exchange for glutamate.
By releasing glutamate, system xc- can potentially cause excitotoxicity.
However, through providing cystine to the cell, it regulates the levels of cellular glutathione (GSH), the main endogenous intracellular antioxidant, and may protect cells against oxidative stress.
We tested two different compounds that deplete primary cortical cultures containing both neurons and astrocytes of intracellular GSH, L-buthionine-sulfoximine (L-BSO), and diethyl maleate (DEM).
Both compounds caused significant concentration and time dependent decreases in intracellular GSH levels.
However; DEM caused an increase in radiolabeled cystine uptake through system xc-, while unexpectedly BSO caused a decrease in uptake.
The compounds caused similar low levels of neurotoxicity, while only BSO caused an increase in oxidative stress.
The mechanism of GSH depletion by these two compounds is different, DEM directly conjugates to GSH, while BSO inhibits γ-glutamylcysteine synthetase, a key enzyme in GSH synthesis.
As would be expected from these mechanisms of action, DEM caused a decrease in intracellular cysteine, while BSO increased cysteine levels.
The results suggest that negative feedback by intracellular cysteine is an important regulator of system xc- in this culture system.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Albano, Rebecca& Raddatz, Nicholas J.& Hjelmhaug, Julie& Baker, David A.& Lobner, Doug. 2015. Regulation of System xc- by Pharmacological Manipulation of Cellular Thiols. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075576
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Albano, Rebecca…[et al.]. Regulation of System xc- by Pharmacological Manipulation of Cellular Thiols. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075576
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Albano, Rebecca& Raddatz, Nicholas J.& Hjelmhaug, Julie& Baker, David A.& Lobner, Doug. Regulation of System xc- by Pharmacological Manipulation of Cellular Thiols. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075576
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075576
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر