![](/images/graphics-bg.png)
Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure
المؤلفون المشاركون
Gwozdzinski, Krzysztof
Pieniazek, Anna
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-18
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The aim of this study was to evaluate the properties of internal components of erythrocytes in chronic renal failure (CRF) patients undergoing hemodialysis (HD) in comparison to control subjects.
For investigation of conformational state of hemoglobin and nonheme proteins (NHP) the maleimide spin label (MSL) in electron paramagnetic resonance (EPR) was applied.
The studies were performed using MSL in whole cells and hemolysate as well as proteins separated by ion exchange chromatography and checked by electrophoresis.
Additionally the level of –SH groups in hemolysate and isolated internal proteins of CRF erythrocytes was determined using 4,4′-dithiodipyridine.
All measurements were performed before and after hemodialysis.
Oxidative stress accompanying CRF/hemodialysed patients caused a significant decrease in the mobility of internal components inside erythrocytes indicated by MSL (P < 0.02).
The significant decrease in mobility of spin labeled HbA1c and HbA both before and after HD (P < 0.0002) as well as in nonheme proteins before hemodialysis (P < 0.05) versus control was indicated.
Decrease in mobility of internal components of erythrocytes was accompanied by loss of thiols before and after hemodialysis versus control in NHP (P < 0.05), HbA1c (P < 0.0002), and HbA (P < 0.0005).
These findings showed oxidative influence of hemodialysis on hemoglobins and internal nonheme proteins in erythrocytes of CRF patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pieniazek, Anna& Gwozdzinski, Krzysztof. 2015. Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075769
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pieniazek, Anna& Gwozdzinski, Krzysztof. Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075769
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pieniazek, Anna& Gwozdzinski, Krzysztof. Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075769
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075769
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)