Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones
المؤلفون المشاركون
Miyoshi, Keiko
Noma, Takafumi
Kondo, Hiroshi
Sakiyama, Shoji
Tangoku, Akira
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-06-17
دولة النشر
مصر
عدد الصفحات
20
الملخص EN
Stem cell therapy appears to be promising for restoring damaged or irreparable lung tissue.
However, establishing a simple and reproducible protocol for preparing lung progenitor populations is difficult because the molecular basis for alveolar epithelial cell differentiation is not fully understood.
We investigated an in vitro system to analyze the regulatory mechanisms of alveolus-specific gene expression using a human alveolar epithelial type II (ATII) cell line, A549.
After cloning A549 subpopulations, each clone was classified into five groups according to cell morphology and marker gene expression.
Two clones (B7 and H12) were further analyzed.
Under serum-free culture conditions, surfactant protein C (SPC), an ATII marker, was upregulated in both H12 and B7.
Aquaporin 5 (AQP5), an ATI marker, was upregulated in H12 and significantly induced in B7.
When the RAS/MAPK pathway was inhibited, SPC and thyroid transcription factor-1 (TTF-1) expression levels were enhanced.
After treatment with dexamethasone (DEX), 8-bromoadenosine 3′5′-cyclic monophosphate (8-Br-cAMP), 3-isobutyl-1-methylxanthine (IBMX), and keratinocyte growth factor (KGF), surfactant protein B and TTF-1 expression levels were enhanced.
We found that A549-derived clones have plasticity in gene expression of alveolar epithelial differentiation markers and could be useful in studying ATII maintenance and differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kondo, Hiroshi& Miyoshi, Keiko& Sakiyama, Shoji& Tangoku, Akira& Noma, Takafumi. 2015. Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones. Stem Cells International،Vol. 2015, no. 2015, pp.1-20.
https://search.emarefa.net/detail/BIM-1076140
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kondo, Hiroshi…[et al.]. Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones. Stem Cells International No. 2015 (2015), pp.1-20.
https://search.emarefa.net/detail/BIM-1076140
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kondo, Hiroshi& Miyoshi, Keiko& Sakiyama, Shoji& Tangoku, Akira& Noma, Takafumi. Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-20.
https://search.emarefa.net/detail/BIM-1076140
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076140
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر