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Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro
المؤلفون المشاركون
Choi, Yeong-Hoon
Neef, Klaus
Wittwer, Thorsten
Wahlers, Thorsten
Treskes, Philipp
Xu, Guoxing
Drey, Florian
Srinivasan, Sureshkumar Perumal
Nembo, Erastus
Semmler, Judith
Nguemo, Filomain
Cowan, Douglas B.
Deppe, Antje-Christin
Scherner, Maximilian
Hescheler, Jürgen
Stamm, Ch.
Šarić, T.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-08-18
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Ischemic heart disease is the main cause of death in western countries and its burden is increasing worldwide.
It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and fatal outcome.
Autologous cells with the potential to regenerate damaged heart tissue would be an ideal source for cell therapeutic approaches.
Here, we compared different methods of conditional culture for increasing the yield and cardiogenic potential of murine skeletal muscle-derived stem cells.
A subpopulation of nonadherent cells was isolated from skeletal muscle by preplating and applying cell culture conditions differing in support of cluster formation.
In contrast to static culture conditions, dynamic culture with or without previous hanging drop preculture led to significantly increased cluster diameters and the expression of cardiac specific markers on the protein and mRNA level.
Whole-cell patch-clamp studies revealed similarities to pacemaker action potentials and responsiveness to cardiac specific pharmacological stimuli.
This data indicates that skeletal muscle-derived stem cells are capable of adopting enhanced cardiac muscle cell-like properties by applying specific culture conditions.
Choosing this route for the establishment of a sustainable, autologous source of cells for cardiac therapies holds the potential of being clinically more acceptable than transgenic manipulation of cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Neef, Klaus& Treskes, Philipp& Xu, Guoxing& Drey, Florian& Srinivasan, Sureshkumar Perumal& Šarić, T.…[et al.]. 2015. Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro. Stem Cells International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1076167
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Neef, Klaus…[et al.]. Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro. Stem Cells International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1076167
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Neef, Klaus& Treskes, Philipp& Xu, Guoxing& Drey, Florian& Srinivasan, Sureshkumar Perumal& Šarić, T.…[et al.]. Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1076167
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076167
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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