Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells
المؤلفون المشاركون
Hopper, Niina
Wardale, John
Howard, Daniel
Brooks, Roger
Rushton, Neil
Henson, Frances
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-01-12
دولة النشر
مصر
عدد الصفحات
9
الملخص EN
A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed.
The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSCs) derived from the infrapatellar fat pad of human OA knee.
Cell migration was measured using an xCELLigence electronic migration chamber system in combination with scratch assays.
Gene expression was quantified with stem cell PCR arrays and validated using quantitative real-time PCR (rtPCR).
In both migration assays PBMCs increased MSC migration by comparison to control.
In scratch assay the wound closure was 55% higher after 3 hours in the PBMC stimulated test group ( P = 0.002 ), migration rate was 9 times faster ( P = 0.008 ), and total MSC migration was 25 times higher after 24 hours ( P = 0.014 ).
Analysis of MSCs by PCR array demonstrated that PBMCs induced the upregulation of genes associated with chondrogenic differentiation over 15-fold.
In conclusion, PBMCs increase both MSC migration and differentiation suggesting that they are an ideal candidate for inclusion in regenerative medicine therapies aimed at cartilage repair.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hopper, Niina& Wardale, John& Howard, Daniel& Brooks, Roger& Rushton, Neil& Henson, Frances. 2015. Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells. Stem Cells International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1076185
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hopper, Niina…[et al.]. Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells. Stem Cells International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1076185
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hopper, Niina& Wardale, John& Howard, Daniel& Brooks, Roger& Rushton, Neil& Henson, Frances. Peripheral Blood Derived Mononuclear Cells Enhance the Migration and Chondrogenic Differentiation of Multipotent Mesenchymal Stromal Cells. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1076185
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076185
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر