Comparisons of Mouse Mesenchymal Stem Cells in Primary Adherent Culture of Compact Bone Fragments and Whole Bone Marrow
المؤلفون المشاركون
Cai, Yiting
Liu, Tianshu
Fang, Fang
Xiong, Chengliang
Shen, Shiliang
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-03
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
The purification of mouse bone marrow mesenchymal stem cells (BMSCs) by using the standard method of whole bone marrow adherence to plastic still remains ineffective.
An increasing number of studies have indicated compact bone as an alternative source of BMSCs.
We isolated BMSCs from cultured compact bone fragments and investigated the proliferative capacity, surface immunophenotypes, and osteogenic and adipogenic differentiations of the cells after the first trypsinization.
The fragment culture was based on the fact that BMSCs were assembled in compact bones.
Thus, the procedure included flushing bone marrow out of bone cavity and culturing the fragments without any collagenase digestion.
The cell yield from cultured fragments was slightly less than that from cultured bone marrow using the same bone quantity.
However, the trypsinized cells from cultured fragments exhibited significantly higher proliferation and were accompanied with more CD90 and CD44 expressions and less CD45 expression.
The osteogenic and adipogenic differentiation capacity of cells from cultured fragments were better than those of cells from bone marrow.
The directly adherent culture of compact bone is suitable for mouse BMSC isolation, and more BMSCs with potentially improved proliferation capacity can be obtained in the primary culture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cai, Yiting& Liu, Tianshu& Fang, Fang& Xiong, Chengliang& Shen, Shiliang. 2015. Comparisons of Mouse Mesenchymal Stem Cells in Primary Adherent Culture of Compact Bone Fragments and Whole Bone Marrow. Stem Cells International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076333
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cai, Yiting…[et al.]. Comparisons of Mouse Mesenchymal Stem Cells in Primary Adherent Culture of Compact Bone Fragments and Whole Bone Marrow. Stem Cells International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076333
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cai, Yiting& Liu, Tianshu& Fang, Fang& Xiong, Chengliang& Shen, Shiliang. Comparisons of Mouse Mesenchymal Stem Cells in Primary Adherent Culture of Compact Bone Fragments and Whole Bone Marrow. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076333
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076333
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر