![](/images/graphics-bg.png)
In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells
المؤلفون المشاركون
Khan, Shaheen N.
Mahmood, Ruhma
Choudhery, Mahmood S.
Mehmood, Azra
Riazuddin, Sheikh
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-01
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Skin autografting is the most viable and aesthetic technique for treatment of extensive burns; however, this practice has potential limitations.
Harvesting cells from neonatal sources (such as placental tissue) is a simple, inexpensive, and noninvasive procedure.
In the current study authors sought to evaluate in vitro potential of human placenta derived stem cells to develop into skin-like cells.
After extensive washing, amniotic membrane and umbilical cord tissue were separated to harvest amniotic epithelial cells (AECs) and umbilical cord mesenchymal stem cells (UC-MSCs), respectively.
Both types of cells were characterized for the expression of embryonic lineage markers and their growth characteristics were determined.
AECs and UC-MSCs were induced to differentiate into keratinocytes-like and dermal fibroblasts-like cells, respectively.
After induction, morphological changes were detected by microscopy.
The differentiation potential was further assessed using immunostaining and RT-PCR analyses.
AECs were positive for cytokeratins and E-Cadherin while UC-MSCs were positive for fibroblast specific makers.
AECs differentiated into keratinocytes-like cells showed positive expression of keratinocyte specific cytokeratins, involucrin, and loricrin.
UC-MSCs differentiated into dermal fibroblast-like cells indicated expression of collagen type 3, desmin, FGF-7, fibroblast activation protein alpha, procollagen-1, and vimentin.
In conclusion, placenta is a potential source of cells to develop into skin-like cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mahmood, Ruhma& Choudhery, Mahmood S.& Mehmood, Azra& Khan, Shaheen N.& Riazuddin, Sheikh. 2015. In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells. Stem Cells International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076365
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mahmood, Ruhma…[et al.]. In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells. Stem Cells International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1076365
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mahmood, Ruhma& Choudhery, Mahmood S.& Mehmood, Azra& Khan, Shaheen N.& Riazuddin, Sheikh. In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076365
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076365
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)