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Beneficial Effects of Coculturing Synovial Derived Mesenchymal Stem Cells with Meniscus Fibrochondrocytes Are Mediated by Fibroblast Growth Factor 1: Increased Proliferation and Collagen Synthesis
المؤلفون المشاركون
Song, Xuanhe
Xie, Yaoping
Liu, Yang
Shao, Ming
Wang, Wenbo
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-16
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Meniscus reconstruction is in great need for orthopedic surgeons.
Meniscal fibrochondrocytes transplantation was proposed to regenerate functional meniscus, with limited donor supply.
We hypothesized that coculture of synovial mesenchymal stem cells (SSC) with meniscal fibrochondrocytes (me-CH) can support matrix production of me-CH, thus reducing the number of me-CH needed for meniscus reconstruction.
A pellet coculture system of human SSC and me-CH was used in this study.
Enhanced glycosaminoglycans (GAG) in coculture pellets were demonstrated by Alcian blue staining and GAG quantification, when compared to monoculture.
More collagen synthesis was shown in coculture pellets by hydroxyproline assay.
Increased proliferation of me-CH was observed in coculture.
Data from BrdU staining and ELISA demonstrated that conditioned medium of SSCs enhanced the proliferation and collagen synthesis of me-CH, and this effect was blocked by neutralizing antibody against fibroblast growth factor 1 (FGF1).
Western blot showed that conditioned medium of SSCs can activate mitogen-activated protein kinase (MAPK) signaling pathways by increasing the phosphorylation of mitogen-activated regulated protein kinase 1/2 (MEK) and extracellular-signal-regulated kinases 1/2 (ERK).
Overall, this study provided evidence that synovial MSCs can support proliferation and collagen synthesis of fibrochondrocytes, by secreting FGF1.
Coimplantation of SSC and me-CH could be a useful strategy for reconstructing meniscus.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Song, Xuanhe& Xie, Yaoping& Liu, Yang& Shao, Ming& Wang, Wenbo. 2015. Beneficial Effects of Coculturing Synovial Derived Mesenchymal Stem Cells with Meniscus Fibrochondrocytes Are Mediated by Fibroblast Growth Factor 1: Increased Proliferation and Collagen Synthesis. Stem Cells International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076386
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Song, Xuanhe…[et al.]. Beneficial Effects of Coculturing Synovial Derived Mesenchymal Stem Cells with Meniscus Fibrochondrocytes Are Mediated by Fibroblast Growth Factor 1: Increased Proliferation and Collagen Synthesis. Stem Cells International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1076386
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Song, Xuanhe& Xie, Yaoping& Liu, Yang& Shao, Ming& Wang, Wenbo. Beneficial Effects of Coculturing Synovial Derived Mesenchymal Stem Cells with Meniscus Fibrochondrocytes Are Mediated by Fibroblast Growth Factor 1: Increased Proliferation and Collagen Synthesis. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076386
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076386
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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