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Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid
المؤلفون المشاركون
Sreeramanan, Subramaniam
Rahmah, Safrina
Ahmad Mubbarakh, Safiah
Soo Ping, Khor
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-11
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
Protocorm-like bodies (PLBs) of Brassidium Shooting Star orchid were successfully cryopreserved using droplet-vitrification method.
Vitrification based cryopreservation protocol is comprised of preculture, osmoprotection, cryoprotection, cooling, rewarming, and growth recovery and each and every step contributes to the achievement of successful cryopreservation.
In order to reveal the lethal and nonlethal damage produced by cryopreservation, histological observation, scanning electron microscopy (SEM), and biochemical analysis were carried out in both cryopreserved and noncryopreserved PLBs of Brassidium Shooting Star orchid comparing with the control PLBs stock culture.
Histological and scanning electron microscopy analyses displayed structural changes in cryopreserved PLBs due to the impact of cryoinjury during exposure to liquid nitrogen.
Total soluble protein significantly increased throughout the dehydration process and the highest value was achieved when PLBs were stored in liquid nitrogen.
Ascorbate peroxidase (APX) and catalase (CAT) showed the highest enzyme activities in both dehydration and cryostorage treatments indicating that stress level of PLBs was high during these stages.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rahmah, Safrina& Ahmad Mubbarakh, Safiah& Soo Ping, Khor& Sreeramanan, Subramaniam. 2015. Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1079326
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rahmah, Safrina…[et al.]. Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid. The Scientific World Journal No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1079326
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rahmah, Safrina& Ahmad Mubbarakh, Safiah& Soo Ping, Khor& Sreeramanan, Subramaniam. Effects of Droplet-Vitrification Cryopreservation Based on Physiological and Antioxidant Enzyme Activities of Brassidium Shooting Star Orchid. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1079326
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1079326
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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