Treatment effect of willow leaves on rheumatoid arthritis in chronic inflammatory disorders of rats

الملخص EN

This Study aimed to determinate some chemical composition of Willow leaves and investigates the treatment effect of Willow leaves powder and extract on injured rheumatoid arthritis in rats injected by 100µl of Complete Freund’s Adjuvant (CFA) into left hind knee joint36 adult male Sprague Dawely rats weighing150-160g classified into control negative(five rats) and rheumatoid arthritis injured rats groups which were control positive group and four treated groups with 10&20% Willow leaves powder and 200 & 400 ppm /kg bw rat /daywillow leave aqueous extract (each five rats).The treatment period was designed for 4weeks .The chemical composition of Willow leaves showed higher value of protein and total dietary fiber in dry weight .Total Phenolic fractionations of Willow illustrated that the salycilic, gentisic, gallic and pyrogallol acids were the major compounds butthe major compoundsoftotal flavonoids compounds were reported thatrutin, hesperidin, naringin, apigenin, and luteolin.

The biological results in positive control grouprevealed that, there was a significant increase in the Malondialdehyde (MDA), creatinine, urea, aspartate amino transferase (ALT&AST) and TNF- alphathere was a significant decrease in the antioxidant enzymes Glutathione Reduced (GSH) and Superoxide activity (SOD) and IL-10compared with control negative control group inclusion of willow leaves powder and Willow leaves extract into the diets of rheumatoid arthritis treated rats improved the levels of all these biomarkers of liver ,kidney ,.antioxidant enzymes, TNF alpha and IL-10 F.

Histopathological examination of the rat knee confirmed this amelioration of health showing that consumption of Willow leaves powder and Willow leaves extract can lower pathological changes in injured rheumatoid arthritis rats.

From the clear results, it could be demonstrated that administration of Willow leaves powder and their extract alleviates the harmful effect of rheumatoid arthritis CFA-induced rats.