Eugenia caryophyllata effect on phenytoin metabolism in animal model

الملخص EN

Phenytoin (PHT) is commonly prescribed antiepileptic drug which is widely used for management of all types of seizure disorders, except absence seizure.

PHT is an inducer of cytochrome P450 (CYP450) CYP2C9 and CYP2C19.

The present study is established to study the effect of Eugenia Caryophyllata (EUC) on PHT pharmacokinetics (PK) in healthy male rabbits.

Materials and Methods: An in-vivo parallel designed PK herb-drug interaction study between EUC and PHT was conducted in healthy male rabbits.

The present study was conducted in two groups (N=6 for each).

In the first group, animals were given orally 15 mg/kg PHT prepared in saline solution and blood samples (1.0 ml) were collected from ear marginal vein at different time intervals (0.5, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 12.0, 24.0, 36.0 and 48 hr) after dosing.

The second group was pretreated with 70 mg/kg/day oily extract from EUC and were given to rabbits for 7 consecutive days.

On the 8th day, PHT was co-administered one hour after the last dose of the EUC oily extract and blood samples were collected at the predetermined intervals as in the first group.

The analysis of PHT blood samples was performed by using PHT detection kit based on chemiluminescent enzyme immunoassay (CLEIA) and different PK parameters such as Cmax, tmax, AUC0-

Results: Our results showed statistically insignificant differences (P>0.05) for the following PK parameters: Cmax, tmax, AUC0-

Conclusion: Our results showed that EUC may not likely to interfere the PK of PHT when co-administered with PHT.

Further confirmation of our findings is required in humans before these results can be applied in patient care.