In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection
المؤلفون المشاركون
Gowrishankar, Shanmugaraj
Karutha Pandian, Shunmugiah
Kamaladevi, Arumugam
Balamurugan, Krishnaswamy
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-28
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The present investigation was deliberately aimed at evaluating the biofilm-forming ability of 63 clinical MRSA isolates recovered from pharyngitis patients through different phenotypic assays.
The molecular detection of adhesion (icaA/icaD/icaB/icaC), adhesins (fnbA/fnbB, clfA, and cna), staphylococcal accessory regulator (sarA), and α-toxin (hla) genes was done by employing polymerase chain reaction (PCR).
Out of 63 isolates, 49 (77.8%) were found slime positive by the Congo red agar (CRA) method and 44 (69.8%) as biofilm positive by the quantitative microtitre plate assays.
The results of MATH assay showed that most of the test pathogens are hydrophilic in nature.
The molecular investigation of biofilm-associated genes revealed that 84.13% ( n = 53 ) of isolates were found positive for icaADBC genes.
The fnbA and fnbB genes were present in 49 (77.8%) and 51 (81%) MRSA isolates, respectively.
In addition, 58.7% ( n = 37 ), 73% ( n = 46 ), and 69.8% ( n = 44 ) of the isolates harboured the clfA, cna, and hla genes, respectively.
Further, nearly 81% ( n = 51 ) of the isolates were found positive for the gene sarA and all the ica negative isolates were also negative for the gene.
Furthermore, the results of in vivo adherence assay unveiled the factual commonness in the in vitro adherence method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gowrishankar, Shanmugaraj& Kamaladevi, Arumugam& Balamurugan, Krishnaswamy& Karutha Pandian, Shunmugiah. 2016. In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection. BioMed Research International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1096845
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gowrishankar, Shanmugaraj…[et al.]. In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection. BioMed Research International No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1096845
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gowrishankar, Shanmugaraj& Kamaladevi, Arumugam& Balamurugan, Krishnaswamy& Karutha Pandian, Shunmugiah. In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1096845
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1096845
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر