Molecular Imaging for Comparison of Different Growth Factors on Bone Marrow-Derived Mesenchymal Stromal Cells’ Survival and Proliferation In Vivo
المؤلفون المشاركون
Li, Congye
Cao, Feng
Qiao, Hongyu
Zhang, Ran
Gao, Lina
Guo, Yanjie
Wang, Jinda
Zhang, Rongqing
Chen, Yundai
Li, Xiujuan
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-21
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Introduction.
Bone marrow-derived mesenchymal stromal cells (BMSCs) have emerged as promising cell candidates but with poor survival after transplantation.
This study was designed to investigate the efficacy of VEGF, bFGF, and IGF-1 on BMSCs’ viability and proliferation both in vivo and in vitro using bioluminescence imaging (BLI).
Methods.
BMSCs were isolated from β-actin-Fluc+ transgenic FVB mice, which constitutively express firefly luciferase.
Apoptosis was induced by hypoxia preconditioning for up to 24 h followed by flow cytometry and TUNEL assay.
106 BMSCs with/without growth factors were injected subcutaneously into wild type FVB mice’s backs.
Survival of BMSCs was longitudinally monitored using bioluminescence imaging (BLI) for 5 weeks.
Protein expression of Akt, p-Akt, PARP, and caspase-3 was detected by Western blot.
Results.
Hypoxia-induced apoptosis was significantly attenuated by bFGF and IGF-1 compared with VEGF and control group in vitro ( P < 0.05 ).
When combined with matrigel, IGF-1 showed the most beneficial effects in protecting BMSCs from apoptosis in vivo.
The phosphorylation of Akt had a higher ratio in the cells from IGF-1 group.
Conclusion.
IGF-1 could protect BMSCs from hypoxia-induced apoptosis through activation of p-Akt/Akt pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qiao, Hongyu& Zhang, Ran& Gao, Lina& Guo, Yanjie& Wang, Jinda& Zhang, Rongqing…[et al.]. 2016. Molecular Imaging for Comparison of Different Growth Factors on Bone Marrow-Derived Mesenchymal Stromal Cells’ Survival and Proliferation In Vivo. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096861
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qiao, Hongyu…[et al.]. Molecular Imaging for Comparison of Different Growth Factors on Bone Marrow-Derived Mesenchymal Stromal Cells’ Survival and Proliferation In Vivo. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096861
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qiao, Hongyu& Zhang, Ran& Gao, Lina& Guo, Yanjie& Wang, Jinda& Zhang, Rongqing…[et al.]. Molecular Imaging for Comparison of Different Growth Factors on Bone Marrow-Derived Mesenchymal Stromal Cells’ Survival and Proliferation In Vivo. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096861
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1096861
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر