Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis
المؤلفون المشاركون
Li, Yu
Ma, Hong
Lu, Ye
Tan, B. J.
Xu, L.
Lawal, Temitope O.
Liu, Daniel
Mahady, Gail B.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-02-14
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The effect of Menoprogen (MPG) on ovarian granulosa cell (GC) apoptosis was investigated in vitro and in vivo in an aged rat model of menopause.
Intragastric administration of Menoprogen or estradiol valerate to 14-month-old senile female rats for eight weeks increased plasma E 2 levels, as well as the weight of both ovarian and uterine tissues.
Flow cytometric (FCM) analysis of isolated GCs from MPG-treated aged rats showed reductions in the G 0 / G 1 ratio and apoptotic peaks.
Isolated GCs also exhibited an increase in cell size and the number of cytoplastic organelles and intracellular gap junctions, the reappearance of secretory granules, and a lack of apoptotic bodies as determined by TEM.
Results from a TdT-mediated dUTP nick end-labeling (TUNEL) assay revealed a reduction in TUNEL-positive GCs after MPG treatment.
Immunohistochemical analysis showed a downregulation of proapoptotic Bax proteins and an upregulation of antiapoptotic Bcl-2 proteins.
The addition of MPG-medicated serum to the media of cultured GCs also reduced cadmium chloride-induced apoptosis and downregulated caspase-3 protein expression.
This work demonstrates that Menoprogen inhibits GC apoptosis in aged female rats and thereby increases E 2 production.
This represents a novel mechanism of action for this herbal medicine in the treatment of menopausal symptoms.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Yu& Ma, Hong& Lu, Ye& Tan, B. J.& Xu, L.& Lawal, Temitope O.…[et al.]. 2016. Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1097106
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Yu…[et al.]. Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1097106
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Yu& Ma, Hong& Lu, Ye& Tan, B. J.& Xu, L.& Lawal, Temitope O.…[et al.]. Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell Apoptosis. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1097106
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097106
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر