Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico

المؤلفون المشاركون

Villa-Mancera, Abel
Molina-Mendoza, Pedro
Hernández-Guzmán, Karina
Olivares-Pérez, Jaime
Sarracent-Pérez, Jorge
Zumaquero-Ríos, José

المصدر

BioMed Research International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-08-01

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الطب البشري

الملخص EN

The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes.

A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA.

Prevalence of 73.46% was found using the serological ELISA and a percentage of 77.20 was found for coproantigen ELISA.

The diagnostic sensitivity and specificity for serum ELISA were 86.7% and 96.4%, and for the coproantigen ELISA they were 93.1% and 97.8%, respectively.

The seropositive samples were further categorized as low, medium, or high positivity.

Results show a great proportion of low and medium positive goats when the serum ELISA test was used.

Correlation coefficients between coproantigens and seropositivity were statistically significant ( P < 0.01 ) for low seropositivity ( r = 0.93 ) and medium seropositivity ( r = 0.84 ).

The accuracy of faecal antigen ELISA was higher compared to indirect ELISA serological test.

Two ELISAs were shown to be useful for demonstrating the current status of F.

hepatica infection in the endemic areas and can be employed in studies on epidemiology as well as anthelmintics treatment for preventing economic loss and the risk of transmission to humans.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Villa-Mancera, Abel& Molina-Mendoza, Pedro& Hernández-Guzmán, Karina& Olivares-Pérez, Jaime& Sarracent-Pérez, Jorge& Zumaquero-Ríos, José. 2016. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097483

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Villa-Mancera, Abel…[et al.]. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1097483

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Villa-Mancera, Abel& Molina-Mendoza, Pedro& Hernández-Guzmán, Karina& Olivares-Pérez, Jaime& Sarracent-Pérez, Jorge& Zumaquero-Ríos, José. Comparative Diagnosis of Serum IgG1 and Coproantigen ELISA for Fasciolosis Detection of Goats in Mexico. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1097483

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1097483