Comparison of Neuroprotective Effect of Bevacizumab and Sildenafil following Induction of Stroke in a Mouse Model

الملخص EN

To evaluate the effect of bevacizumab and sildenafil on stroke parameters in a mouse model, middle cerebral artery occlusion was induced in male C57Bl/6 mice using an intra-arterial filament method.

The filament was removed after 60 minutes, and the mice were immediately given a single intraperitoneal injection of saline, bevacizumab, or sildenafil.

An additional group of mice (n=7) received bevacizumab 6 h after MCAO induction.

The mice were euthanized 24 hours later and evaluated for infarct area and brain edema using triphenyltetrazolium chloride staining and ImageJ.

In the saline-treated mice (n=16), total stroke volume was 19.20±6.38 mm3, mean penumbra area was 4.5±2.03 mm3, and hemispheric asymmetry was 106.5%.

Corresponding values in the bevacizumab group (n=19) were 17.79±5.80 mm3, 7.3±3.5 mm3, and 108.6%; in the delayed (6 h) bevacizumab injected mice (n=7) they were 9.80±8.00 mm3, 2.4±2.0 mm3, and 98.2%; and in the sildenafil group (n=16) they were 18.42±5.41 mm3, 5.7±2.02 mm3, and 109.9%.

The bevacizumab group had a significantly larger mean penumbra area when given immediately and smaller total stroke area in both groups than the saline- (p=0.03) and sildenafil-treated (p=0.003) groups.

Only delayed bevacizumab group had reduced edema.

Bevacizumab, injected immediately or delayed after injury, exerts a neuroprotective/salvage effect, whereas immediate treatment with sildenafil does not.

Inflammation may play a role in the neuroprotective effect.