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RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism
المؤلفون المشاركون
Ma, Liang
Chen, Ling
Zhang, Lei
Zou, Gen
Liu, Rui
Jiang, Yanping
Zhou, Zhihua
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-27
دولة النشر
مصر
عدد الصفحات
20
التخصصات الرئيسية
الملخص EN
Xyr1 has been demonstrated to be the main transcription activator of (hemi)cellulases in the well-known cellulase producer Trichoderma reesei.
This study comprehensively investigates the genes regulated by Xyr1 through RNA sequencing to produce the transcription profiles of T.
reesei Rut-C30 and its xyr1 deletion mutant (Δxyr1), cultured on lignocellulose or glucose.
xyr1 deletion resulted in 467 differentially expressed genes on inducing medium.
Almost all functional genes involved in (hemi)cellulose degradation and many transporters belonging to the sugar porter family in the major facilitator superfamily (MFS) were downregulated in Δxyr1.
By contrast, all differentially expressed protease, lipase, chitinase, some ATP-binding cassette transporters, and heat shock protein-encoding genes were upregulated in Δxyr1.
When cultured on glucose, a total of 281 genes were expressed differentially in Δxyr1, most of which were involved in energy, solute transport, lipid, amino acid, and monosaccharide as well as secondary metabolism.
Electrophoretic mobility shift assays confirmed that the intracellular β-glucosidase bgl2, the putative nonenzymatic cellulose-attacking gene cip1, the MFS lactose transporter lp, the nmrA-like gene, endo T, the acid protease pepA, and the small heat shock protein hsp23 were probable Xyr1-targets.
These results might help elucidate the regulation system for synthesis and secretion of (hemi)cellulases in T.
reesei Rut-C30.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ma, Liang& Chen, Ling& Zhang, Lei& Zou, Gen& Liu, Rui& Jiang, Yanping…[et al.]. 2016. RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism. BioMed Research International،Vol. 2016, no. 2016, pp.1-20.
https://search.emarefa.net/detail/BIM-1097899
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ma, Liang…[et al.]. RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism. BioMed Research International No. 2016 (2016), pp.1-20.
https://search.emarefa.net/detail/BIM-1097899
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ma, Liang& Chen, Ling& Zhang, Lei& Zou, Gen& Liu, Rui& Jiang, Yanping…[et al.]. RNA Sequencing Reveals Xyr1 as a Transcription Factor Regulating Gene Expression beyond Carbohydrate Metabolism. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-20.
https://search.emarefa.net/detail/BIM-1097899
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097899
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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