FBS or BSA Inhibits EGCG Induced Cell Death through Covalent Binding and the Reduction of Intracellular ROS Production
المؤلفون المشاركون
Shen, Han-Ming
Zhang, Yin
Xu, Yu-Ying
Sun, Wen-Jie
Zhang, Mo-Han
Zheng, Yi-Fan
Yang, Jun
Zhu, Xin-Qiang
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Previously we have shown that ( - )-epigallocatechin gallate (EGCG) can induce nonapoptotic cell death in human hepatoma HepG2 cells only under serum-free condition.
However, the underlying mechanism for serum in determining the cell fate remains to be answered.
The effects of fetal bovine serum (FBS) and its major component bovine serum albumin (BSA) on EGCG-induced cell death were investigated in this study.
It was found that BSA, just like FBS, can protect cells from EGCG-induced cell death in a dose-dependent manner.
Detailed analysis revealed that both FBS and BSA inhibited generation of ROS to protect against toxicity of EGCG.
Furthermore, EGCG was shown to bind to certain cellular proteins including caspase-3, PARP, and α-tubulin, but not LC3 nor β-actin, which formed EGCG-protein complexes that were inseparable by SDS-gel.
On the other hand, addition of FBS or BSA to culture medium can block the binding of EGCG to these proteins.
In silico docking analysis results suggested that BSA had a stronger affinity to EGCG than the other proteins.
Taken together, these data indicated that the protective effect of FBS and BSA against EGCG-induced cell death could be due to (1) the decreased generation of ROS and (2) the competitive binding of BSA to EGCG.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yin& Xu, Yu-Ying& Sun, Wen-Jie& Zhang, Mo-Han& Zheng, Yi-Fan& Shen, Han-Ming…[et al.]. 2016. FBS or BSA Inhibits EGCG Induced Cell Death through Covalent Binding and the Reduction of Intracellular ROS Production. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097975
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yin…[et al.]. FBS or BSA Inhibits EGCG Induced Cell Death through Covalent Binding and the Reduction of Intracellular ROS Production. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1097975
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yin& Xu, Yu-Ying& Sun, Wen-Jie& Zhang, Mo-Han& Zheng, Yi-Fan& Shen, Han-Ming…[et al.]. FBS or BSA Inhibits EGCG Induced Cell Death through Covalent Binding and the Reduction of Intracellular ROS Production. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097975
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097975
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر