Quantitative In Vitro and In Vivo Evaluation of Intestinal and Blood-Brain Barrier Transport Kinetics of the Plant N-Alkylamide Pellitorine
المؤلفون المشاركون
Veryser, Lieselotte
Bracke, Nathalie
Wynendaele, Evelien
Joshi, Tanmayee
Tatke, Pratima
Taevernier, Lien
De Spiegeleer, Bart
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-04
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Objective.
To evaluate the gut mucosa and blood-brain barrier (BBB) pharmacokinetic permeability properties of the plant N-alkylamide pellitorine.
Methods.
Pure pellitorine and an Anacyclus pyrethrum extract were used to investigate the permeation of pellitorine through (1) a Caco-2 cell monolayer, (2) the rat gut after oral administration, and (3) the BBB in mice after intravenous and intracerebroventricular administration.
A validated bioanalytical UPLC-MS2 method was used to quantify pellitorine.
Results.
Pellitorine was able to cross the Caco-2 cell monolayer from the apical-to-basolateral and from the basolateral-to-apical side with apparent permeability coefficients between 0.6 · 10 - 5 and 4.8 · 10 - 5 cm/h and between 0.3 · 10 - 5 and 5.8 · 10 - 5 cm/h, respectively.
In rats, a serum elimination rate constant of 0.3 h−1 was obtained.
Intravenous injection of pellitorine in mice resulted in a rapid and high permeation of pellitorine through the BBB with a unidirectional influx rate constant of 153 μL/(g·min).
In particular, 97% of pellitorine reached the brain tissue, while only 3% remained in the brain capillaries.
An efflux transfer constant of 0.05 min−1 was obtained.
Conclusion.
Pellitorine shows a good gut permeation and rapidly permeates the BBB once in the blood, indicating a possible role in the treatment of central nervous system diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Veryser, Lieselotte& Bracke, Nathalie& Wynendaele, Evelien& Joshi, Tanmayee& Tatke, Pratima& Taevernier, Lien…[et al.]. 2016. Quantitative In Vitro and In Vivo Evaluation of Intestinal and Blood-Brain Barrier Transport Kinetics of the Plant N-Alkylamide Pellitorine. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098146
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Veryser, Lieselotte…[et al.]. Quantitative In Vitro and In Vivo Evaluation of Intestinal and Blood-Brain Barrier Transport Kinetics of the Plant N-Alkylamide Pellitorine. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1098146
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Veryser, Lieselotte& Bracke, Nathalie& Wynendaele, Evelien& Joshi, Tanmayee& Tatke, Pratima& Taevernier, Lien…[et al.]. Quantitative In Vitro and In Vivo Evaluation of Intestinal and Blood-Brain Barrier Transport Kinetics of the Plant N-Alkylamide Pellitorine. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098146
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098146
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر